FCM/FACS (Flow Cytometry)
(CAD Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit CAD Polyclonal Antibody | anti-CAD antibody
CAD Antibody (Center)
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CAD, Antibody; CAD Antibody (Center); CAD protein; Glutamine-dependent carbamoyl-phosphate synthase; Aspartate carbamoyltransferase; Dihydroorotase; CAD; anti-CAD antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 780-809 amino acids from the Central region of human CAD.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.29 (varies by lot)
Sequence Positions
780-809
Sequence Length
2,225
Applicable Applications for anti-CAD antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CAD Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CAD Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(CAD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(CAD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(CAD Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CAD antibody detected the CAD protein (arrow).)
WB (Western Blot)
(CAD Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CAD antibody detected the CAD protein (arrow).)
Related Product Information for anti-CAD antibody
The de novo synthesis of pyrimidine nucleotides is
required for mammalian cells to proliferate. This gene encodes a
trifunctional protein which is associated with the enzymatic
activities of the first 3 enzymes in the 6-step pathway of
pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II),
aspartate transcarbamoylase, and dihydroorotase. This protein is
regulated by the mitogen-activated protein kinase (MAPK) cascade,
which indicates a direct link between activation of the MAPK
cascade and de novo biosynthesis of pyrimidine nucleotides.
required for mammalian cells to proliferate. This gene encodes a
trifunctional protein which is associated with the enzymatic
activities of the first 3 enzymes in the 6-step pathway of
pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II),
aspartate transcarbamoylase, and dihydroorotase. This protein is
regulated by the mitogen-activated protein kinase (MAPK) cascade,
which indicates a direct link between activation of the MAPK
cascade and de novo biosynthesis of pyrimidine nucleotides.
Product Categories/Family for anti-CAD antibody
References
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Ahuja, V., et al. J. Inherit. Metab. Dis. 31(4):481-491(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Ahuja, V., et al. J. Inherit. Metab. Dis. 31(4):481-491(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
242984
NCBI Official Full Name
CAD protein isoform 1
NCBI Official Synonym Full Names
carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
NCBI Official Symbol
CAD
NCBI Protein Information
CAD protein
UniProt Protein Name
CAD protein
UniProt Gene Name
CAD
UniProt Entry Name
PYR1_HUMAN
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Product Notes
The CAD cad (Catalog #AAA287846) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 780-809. The CAD Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's CAD can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CAD cad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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