WB (Western Blot)
(Western blot analysis of Rat testis cell lysate (25ug) probed with Rabbit anti Rat Calcitonin Receptor (AAA50476) at a 1/500 dilution (Lane A) or no primary antibody (Lane B) followed by detection with HRP conjugated Goat anti Rabbit IgG (H/L) at a 1/10,000 dilution and visualized on the ChemiDoc MP imaging system. Performed under reduced conditions. Arrows point to Rat Calcitonin Receptor (molecular weights 70 and 50 kDa).)
Rabbit CALCITONIN RECEPTOR Polyclonal Antibody | anti-Calcr antibody
RABBIT ANTI RAT CALCITONIN RECEPTOR
Reactivity
Reacts with: MouseReacts weakly with: Human
Antibody reactivity and working conditions may vary between species.
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Antisera to calcitonin receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Synonyms
CALCITONIN RECEPTOR, Antibody; RABBIT ANTI RAT CALCITONIN RECEPTOR; anti-Calcr antibody
Host
Rabbit
Reactivity
Reacts with: Mouse
Reacts weakly with: Human
Antibody reactivity and working conditions may vary between species.
Reacts weakly with: Human
Antibody reactivity and working conditions may vary between species.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antisera to calcitonin receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Form/Format
Purified IgG - lyophilized
10 mM Sodium Phosphate
0.01% Sodium Azide (NaN3)
10 mM Sodium Phosphate
0.01% Sodium Azide (NaN3)
Concentration
IgG concentration 1.0mg/ml after reconstitution (varies by lot)
Applicable Applications for anti-Calcr antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Synthetic peptide derived from a C-terminal sequence of rat calcitonin receptor.
Reconstitution
Reconstitute with 100ul distilled water
Positive Control Tissue
Kidney, cultured osteoclasts
Target Species
Rat
Preparation and Storage
Prior to reconstitution store at 4°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
WB (Western Blot)
(Western blot analysis of Rat testis cell lysate (25ug) probed with Rabbit anti Rat Calcitonin Receptor (AAA50476) at a 1/500 dilution (Lane A) or no primary antibody (Lane B) followed by detection with HRP conjugated Goat anti Rabbit IgG (H/L) at a 1/10,000 dilution and visualized on the ChemiDoc MP imaging system. Performed under reduced conditions. Arrows point to Rat Calcitonin Receptor (molecular weights 70 and 50 kDa).)
WB (Western Blot)
(Western blot analysis of Rat testis cell lysate (25ug) probed with Rabbit anti Rat Calcitonin Receptor (AAA50476) at a 1/500 dilution (Lane A) or no primary antibody (Lane B) followed by detection with HRP conjugated Goat anti Rabbit IgG (H/L) at a 1/10,000 dilution and visualized on the ChemiDoc MP imaging system. Performed under reduced conditions. Arrows point to Rat Calcitonin Receptor (molecular weights 70 and 50 kDa).)
Related Product Information for anti-Calcr antibody
Rabbit anti rat calcitonin receptor antibody recognizes rat calcitonin receptor, also known as CT-R or C1A/C1B.Calcitonin receptor is a seven-transmembrane domain G protein-coupled receptor that binds the protein hormone calcitonin. It is expressed in kidneys, central and peripheral nervous system, skeletal muscle stem cells and osteocytes. Investigations on calcitonin receptor's function have mainly focused on its role in regulating calcium homeostasis and osteoclast-mediated bone resorption. However, a growing body of evidence suggests that calcitonin receptor could also be involved in foetal morphogenesis and cancer development.Rabbit anti rat calcitonin receptor antibody binds to an intracellular epitope of the calcitonin receptor that is shared by both C1a and C1b rat calcitonin receptor isoforms. It has been shown to cross-react with mouse calcitonin receptor.
Product Categories/Family for anti-Calcr antibody
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Product Notes
The Calcr (Catalog #AAA50476) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI RAT CALCITONIN RECEPTOR reacts with Reacts with: Mouse Reacts weakly with: Human Antibody reactivity and working conditions may vary between species. and may cross-react with other species as described in the data sheet. AAA Biotech's CALCITONIN RECEPTOR can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Calcr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CALCITONIN RECEPTOR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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