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IF (Immunofluorescence)
(AAA323184 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA323184 1:200) and mouse anti-beta tubulin Ab (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab (Green) were used as the secondary Ab.)
Rabbit CALCOCO2 Polyclonal Antibody | anti-CALCOCO2 antibody
CALCOCO2 Antibody
Gene Names
CALCOCO2; NDP52
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CALCOCO2, Antibody; CALCOCO2 Antibody; anti-CALCOCO2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
CALCOCO2 antibody detects endogenous levels of CALCOCO2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
470
Applicable Applications for anti-CALCOCO2 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CALCOCO2.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA323184 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA323184 1:200) and mouse anti-beta tubulin Ab (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab (Green) were used as the secondary Ab.)
IF (Immunofluorescence)
(AAA323184 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA323184 1:200) and mouse anti-beta tubulin Ab (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab (Green) were used as the secondary Ab.)
WB (Western Blot)
(Western blot analysis of extracts of rat skeletal muscle tissue, using CALCOCO2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of rat skeletal muscle tissue, using CALCOCO2 antibody.)
IHC (Immunohiostchemistry)
(AAA323184 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA323184 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts from 293, using CALCOCO2 antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from 293, using CALCOCO2 antibody. Lane 1 was treated with the blocking peptide.)
Related Product Information for anti-CALCOCO2 antibody
Function: Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy (PubMed:22246324). Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation (PubMed:23022382, PubMed:25771791). Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C (PubMed:23022382, PubMed:25771791). May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion (PubMed:17635994).
Similarity: The MYO6-binding domain is required for autophagy-mediated degradation of infecting bacteria such as Salmonella typhimurium, but not for bacteria targeting to autophagosomes.The CLIR (LC3C-interacting region) motif is required for interaction with MAP1LC3C, but dispensable for CALCOCO2-mediated autophagosome maturation.The LIR-like motif is required for interaction with MAP1LC3A, MAP1LC3B and GABARAPL2, as well as for CALCOCO2-mediated autophagosome maturation.The LGALS8-binding domain is essential for the recruitment to cytosol-exposed infecting bacteria. Belongs to the CALCOCO family.
Similarity: The MYO6-binding domain is required for autophagy-mediated degradation of infecting bacteria such as Salmonella typhimurium, but not for bacteria targeting to autophagosomes.The CLIR (LC3C-interacting region) motif is required for interaction with MAP1LC3C, but dispensable for CALCOCO2-mediated autophagosome maturation.The LIR-like motif is required for interaction with MAP1LC3A, MAP1LC3B and GABARAPL2, as well as for CALCOCO2-mediated autophagosome maturation.The LGALS8-binding domain is essential for the recruitment to cytosol-exposed infecting bacteria. Belongs to the CALCOCO family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 52 kDa
NCBI Official Full Name
calcium-binding and coiled-coil domain-containing protein 2 isoform 1
NCBI Official Synonym Full Names
calcium binding and coiled-coil domain 2
NCBI Official Symbol
CALCOCO2
NCBI Official Synonym Symbols
NDP52
NCBI Protein Information
calcium-binding and coiled-coil domain-containing protein 2
UniProt Protein Name
Calcium-binding and coiled-coil domain-containing protein 2
UniProt Gene Name
CALCOCO2
UniProt Synonym Gene Names
NDP521 PublicationManual assertion based on opinion iniRef.1; Nuclear domain 10 protein 52
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Product Notes
The CALCOCO2 calcoco2 (Catalog #AAA323184) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CALCOCO2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CALCOCO2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CALCOCO2 calcoco2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CALCOCO2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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