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Application Data
(Human A431 cell was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100,then stained with 20ug/ml rabbit Anti-human CALML5 Polyclonal Antibody (AAA21028, red histogram) or Isotype control antibody (Catalog , green histogram), followed by 1ug/ml FITC conjugated Anti-rabbit IgG Secondary Antibody)
Rabbit anti-Human Calmodulin Like Protein 5 Polyclonal Antibody | anti-CALML5 antibody
Calmodulin Like Protein 5 (CALML5) Polyclonal Antibody
Gene Names
CALML5; CLSP
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Calmodulin Like Protein 5, Antibody; Calmodulin Like Protein 5 (CALML5) Polyclonal Antibody; CLSP; Calmodulin-like skin protein; anti-CALML5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.5mg/mL (varies by lot)
Applicable Applications for anti-CALML5 antibody
Western Blot (WB); Immunohistochemistry (IHC).
Application Notes
Western blotting: 0.01-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant CALML5 (Met1~Glu146) expressed in E.coli
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Application Data
(Human A431 cell was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100,then stained with 20ug/ml rabbit Anti-human CALML5 Polyclonal Antibody (AAA21028, red histogram) or Isotype control antibody (Catalog , green histogram), followed by 1ug/ml FITC conjugated Anti-rabbit IgG Secondary Antibody)
Application Data
(Human A431 cell was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100,then stained with 20ug/ml rabbit Anti-human CALML5 Polyclonal Antibody (AAA21028, red histogram) or Isotype control antibody (Catalog , green histogram), followed by 1ug/ml FITC conjugated Anti-rabbit IgG Secondary Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Human Breast cancer TissuePrimary Ab: 10ug/ml Rabbit Anti-Human CALML5 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Human Breast cancer TissuePrimary Ab: 10ug/ml Rabbit Anti-Human CALML5 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Breast cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human CALML5 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Breast cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human CALML5 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Skin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human CALML5 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Skin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human CALML5 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Sample: Recombinant CALML5, Human.)
WB (Western Blot)
(Sample: Recombinant CALML5, Human.)
WB (Western Blot)
(Sample: Human A431 cell lysate;Primary Ab: 1ug/ml Rabbit Anti-Human CALML5 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Sample: Human A431 cell lysate;Primary Ab: 1ug/ml Rabbit Anti-Human CALML5 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Product Categories/Family for anti-CALML5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
calmodulin-like protein 5
NCBI Official Synonym Full Names
calmodulin like 5
NCBI Official Symbol
CALML5
NCBI Official Synonym Symbols
CLSP
NCBI Protein Information
calmodulin-like protein 5
UniProt Protein Name
Calmodulin-like protein 5
UniProt Gene Name
CALML5
UniProt Synonym Gene Names
CLSP
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Product Notes
The CALML5 calml5 (Catalog #AAA21028) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calmodulin Like Protein 5 (CALML5) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calmodulin Like Protein 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC). Western blotting: 0.01-2ug/mL; Immunohistochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CALML5 calml5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calmodulin Like Protein 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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