Rabbit anti-Human, Rat Calponin-1 Polyclonal Antibody | anti-CNN1 antibody

Calponin-1 (Smooth Muscle Marker)

Cat. Num. AAA214898

• Gene Names: CNN1; SMCC; Sm-Calp; HEL-S-14
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Calponin-1, Antibody; Calponin-1 (Smooth Muscle Marker); Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; Calponin-1; CNN1; Cnn1; Sm Calp; SMCC; anti-CNN1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this antibody reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 297

• Applicable Applications for anti-CNN1 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Positive Control: Myoepithelial cells in breast ducts.
• Immunogen: Recombinant human CNN1 protein
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 19p13.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded Rat Uterus stained with Calponin Rabbit Polyclonal Antibody)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Uterus stained with Calponin Rabbit Polyclonal Antibody)

References

Tang, D.C., et al. 1996. Structure-function relations of smooth muscle Calponin. The critical role of Serine 175. J. Biol. Chem. 271: 8605-8611.

NCBI and Uniprot Product Information

• NCBI GI #: 21361120
• NCBI GeneID: 1264
• NCBI Accession #: NP_001290.2
• NCBI GenBank Nucleotide #: NP_001290.2
• UniProt Accession #: P51911
• Molecular Weight: 34kDa
• NCBI Official Full Name: calponin-1 isoform 1
• NCBI Official Synonym Full Names: calponin 1
• NCBI Official Symbol: CNN1
• NCBI Official Synonym Symbols: SMCC; Sm-Calp; HEL-S-14
• NCBI Protein Information: calponin-1
• UniProt Protein Name: Calponin-1
• UniProt Gene Name: CNN1
• UniProt Entry Name: CNN1_HUMAN