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WB (Western Blot)
(Anti-CAMKIV rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:52 kDaObserved band size:59 kDa)
Rabbit anti-Mouse CAMKIV Polyclonal Antibody | anti-CAMK4 antibody
Anti-CAMKIV Antibody, Rabbit Polyclonal
Gene Names
Camk4; CaMKIV; AI666733; CaMKIV/Gr; D18Bwg0362e; A430110E23Rik
Reactivity
Mouse
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CAMKIV, Antibody; Anti-CAMKIV Antibody, Rabbit Polyclonal; CAMKIV Antibody, Rabbit PAb, Antigen Affinity Purified; calcium/calmodulin-dependent protein kinase IV; Anti-A430110E23Rik Antibody; Anti-Camk4 Antibody; Anti-CaMKIV Antibody; Anti-CaMKIV/Gr Antibody; Anti-D18Bwg0362e Antibody; anti-CAMK4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse CAMKIV
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CAMK4 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse CAMKIV Protein ( P08414; Met1-Tyr469). CAMKIV specific IgG was purified by Mouse CAMKIV affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
WB (Western Blot)
(Anti-CAMKIV rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:52 kDaObserved band size:59 kDa)
WB (Western Blot)
(Anti-CAMKIV rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:52 kDaObserved band size:59 kDa)
IP (Immunoprecipitation)
(CAMK4 was immunoprecipitated using: Lane A:0.5 mg Mouse brain Whole Cell Lysate 4 uL anti-CAMK4 rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CAMK4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 52 kDa Observed band size :59 kDa)
IP (Immunoprecipitation)
(CAMK4 was immunoprecipitated using: Lane A:0.5 mg Mouse brain Whole Cell Lysate 4 uL anti-CAMK4 rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CAMK4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 52 kDa Observed band size :59 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of Mouse CAMKIV in Mouse testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of Mouse CAMKIV in Mouse testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of Mouse CAMKIV in Mouse brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of Mouse CAMKIV in Mouse brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-CAMK4 antibody
Ca2+/calmodulin-dependent protein kinase 4 (CAMKIV) belongs to the serine/threonine protein kinase family, and to the Ca2+/calmodulin-dependent protein kinase subfamily which is widely recognized as an essential enzyme implicated in the phophoinositide amplification cascade. Ca2+/calmodulin dependent protein kinase (CAMK) can be activated by the introcellular increased Ca2+ and then apt to combine with the target protein. Ca2+/calmodulin-dependent protein kinase 4 (CAMKIV) is a multifunctional CaM-dependent kinase protein with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. All of the isforms of this family, including myosin light chain kinase, phosphorylase kinase, CaMK1, CaMKIII and CaMKIV have EF-hand structure.
Product Categories/Family for anti-CAMK4 antibody
References
Felician. DM. e. al. (2009. Repressio. o. Ca2+/calmodulin-dependen. protei. kinas. I. signalin. accelerate. retinoi. acid-induce. differentiatio. o. huma. neuroblastom. cells. . Bio. Chem. 28. (39). 26466-81.Zha. X. e. al. (2001). Th. modula. natur. o. histon. deacetylas. HDAC. confer. phosphorylation-dependen. intracellula. trafficking. . Bio. Chem. 27. (37). 35042-8.Raciopp. L. e. al. (2008. Calcium/calmodulin-dependen. kinas. I. i. immun. an. inflammator. responses. nove. route. fo. a. ancien. traveller.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,628 Da
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type IV
NCBI Official Synonym Full Names
calcium/calmodulin-dependent protein kinase IV
NCBI Official Symbol
Camk4
NCBI Official Synonym Symbols
CaMKIV; AI666733; CaMKIV/Gr; D18Bwg0362e; A430110E23Rik
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type IV
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type IV
UniProt Gene Name
Camk4
UniProt Synonym Gene Names
CaMK IV
UniProt Entry Name
KCC4_MOUSE
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Product Notes
The CAMK4 camk4 (Catalog #AAA259533) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CAMKIV Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CAMKIV can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CAMK4 camk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAMKIV, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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