WB (Western Blot)
(Detection of human CAP-D3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, MOLT-4, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-D3 antibody (AAA211047 lot 5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human CAP-D3 Polyclonal Antibody | anti-CAP-D3 antibody
Rabbit anti-CAP-D3 Antibody, Affinity Purified
Gene Names
NCAPD3; CAPD3; hcp-6; CAP-D3; hHCP-6; hCAP-D3
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CAP-D3, Antibody; Rabbit anti-CAP-D3 Antibody, Affinity Purified; hcp-6; Non-SMC condensin II complex subunit D3; MCPH22; hHCP-6; hCAP-D3; condensin-2 complex subunit D3; CAPD3; CAP-D3; Condensin-2 complex subunit D3; non-SMC condensin II complex subunit D3; anti-CAP-D3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-CAP-D3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1448 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CAP-D3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, MOLT-4, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-D3 antibody (AAA211047 lot 5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human CAP-D3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, MOLT-4, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-D3 antibody (AAA211047 lot 5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CAP-D3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-D3 antibody (AAA211047 lot 5) used for IP at 6 ug per reaction. CAP-D3 was also immunoprecipitated by a previous lot of this antibody (AAA211047 lot 4) and a second antibody against a different epitope of CAP-D3 (BL2943). For blotting immunoprecipitated CAP-D3, AAA211047 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CAP-D3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAP-D3 antibody (AAA211047 lot 5) used for IP at 6 ug per reaction. CAP-D3 was also immunoprecipitated by a previous lot of this antibody (AAA211047 lot 4) and a second antibody against a different epitope of CAP-D3 (BL2943). For blotting immunoprecipitated CAP-D3, AAA211047 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-CAP-D3 antibody
CAP-D3 is a subunit of the heteropentameric condensin II complex. Condensin participates in the condensation of chromosomes during cell division by facilitating positive DNA supercoiling and knotting by topoisomerase I and II, and aids in the proper segregation of sister chromatids. In the condensin II complex CAP-D3 is regulated by protein phosphatase 2A (PP2A) and may participate in centromere resolution.
Product Categories/Family for anti-CAP-D3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
168,891 Da
NCBI Official Full Name
condensin-2 complex subunit D3
NCBI Official Synonym Full Names
non-SMC condensin II complex, subunit D3
NCBI Official Symbol
NCAPD3
NCBI Official Synonym Symbols
CAPD3; hcp-6; CAP-D3; hHCP-6; hCAP-D3
NCBI Protein Information
condensin-2 complex subunit D3
UniProt Protein Name
Condensin-2 complex subunit D3
UniProt Gene Name
NCAPD3
UniProt Synonym Gene Names
CAPD3; KIAA0056; hCAP-D3
UniProt Entry Name
CNDD3_HUMAN
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Product Notes
The CAP-D3 ncapd3 (Catalog #AAA211047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CAP-D3 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAP-D3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CAP-D3 ncapd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAP-D3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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