IF (Immunofluorescence)
(Immunofluorescence staining of CXADR in HepG2 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human CXADR polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.)
Rabbit anti-Human CAR/CXADR Polyclonal Antibody | anti-CXADR antibody
Anti-CAR/CXADR Antibody, Rabbit Polyclonal
Gene Names
CXADR; CAR; HCAR; CAR4/6
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
CAR/CXADR, Antibody; Anti-CAR/CXADR Antibody, Rabbit Polyclonal; CXADR/CAR Antibody, Rabbit PAb, Antigen Affinity Purified; coxsackie virus and adenovirus receptor; Anti-CAR Antibody; Anti-CAR4/6 Antibody; Anti-CXADR Antibody; Anti-HCAR Antibody; anti-CXADR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CXADR/CAR
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CXADR antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA
Immunogen
Recombinant Human CXADR/CAR Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human CXADR/CAR (rh CXADR/CAR). CXADR/CAR specific IgG was purified by Human CXADR/CAR affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of CXADR in HepG2 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human CXADR polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.)
IF (Immunofluorescence)
(Immunofluorescence staining of CXADR in HepG2 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human CXADR polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CXADR in human bladder carcinoma with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CXADR in human bladder carcinoma with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-CXADR antibody
CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 17 aa intracellular domain, among which, D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 9% sequence identity with the corresponding regions of rat, human CXADR.
References
Tomko, R.P. et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94 (7): 3352-3356. van Raaij, M.J. et al., 2001, Structure. 8 (11): 1147-1155. Cohen, C.J. et al., 2001, J. Biol. Chem. 276 (27): 25392-25398. Carson, S.D. et al., 2002, Rev. Med. Virol. 11 (4): 219-226. Selinka, H.C. et al., 2004, Med. Microbiol. Immunol. 193 (2-3): 127-131. Philipson, L. et al., 2004, Curr. Top. Microbiol. Immunol. 273:87-111. Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-1580.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,570 Da
NCBI Official Full Name
coxsackievirus and adenovirus receptor isoform 1
NCBI Official Synonym Full Names
coxsackie virus and adenovirus receptor
NCBI Official Symbol
CXADR
NCBI Official Synonym Symbols
CAR; HCAR; CAR4/6
NCBI Protein Information
coxsackievirus and adenovirus receptor; HCVADR; CVB3-binding protein; coxsackievirus B-adenovirus receptor; 46 kD coxsackievirus and adenovirus receptor (CAR) protein
UniProt Protein Name
Coxsackievirus and adenovirus receptor
UniProt Gene Name
CXADR
UniProt Synonym Gene Names
CAR; CAR; hCAR
UniProt Entry Name
CXAR_HUMAN
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Product Notes
The CXADR cxadr (Catalog #AAA255168) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CAR/CXADR Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAR/CXADR can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the CXADR cxadr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAR/CXADR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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