<
WB (Western Blot)
(Immunoprecipitating CA1 in K562 whole cell lysateLane 1: Rabbit control IgG instead of AAA26929 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26929 (8ug) + K562 whole cell lysate (500ug)Lane 3: K562 whole cell lysate (20ug))
Rabbit anti-Human, Mouse Carbonic anhydrase 1 Polyclonal Antibody | anti-CA1 antibody
Rabbit anti-human Carbonic anhydrase 1 polyclonal Antibody
Gene Names
CA1; CAB; CA-I; Car1; HEL-S-11
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
>95%, Protein G purified
Synonyms
Carbonic anhydrase 1, Antibody; Rabbit anti-human Carbonic anhydrase 1 polyclonal Antibody; Carbonic anhydrase 1 (EC 4.2.1.1) (Carbonate dehydratase I) (Carbonic anhydrase B) (CAB) (Carbonic anhydrase I) (CA-I), CA1; anti-CA1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
261
Applicable Applications for anti-CA1 antibody
ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
Immunogen
Recombinant Human Carbonic anhydrase 1 protein (2-261AA)
Species
Human
Research Area
Cardiovascular
Target Names
CA1
Conjugate
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-50895 / sc-50898 / sc-50899 / sc-134853
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
WB (Western Blot)
(Immunoprecipitating CA1 in K562 whole cell lysateLane 1: Rabbit control IgG instead of AAA26929 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26929 (8ug) + K562 whole cell lysate (500ug)Lane 3: K562 whole cell lysate (20ug))
WB (Western Blot)
(Immunoprecipitating CA1 in K562 whole cell lysateLane 1: Rabbit control IgG instead of AAA26929 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26929 (8ug) + K562 whole cell lysate (500ug)Lane 3: K562 whole cell lysate (20ug))
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysate, Mouse spleen tissue, Mouse kidney tissueAll lanes: CA1 antibody at 6.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29 kDaObserved band size: 29 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysate, Mouse spleen tissue, Mouse kidney tissueAll lanes: CA1 antibody at 6.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29 kDaObserved band size: 29 kDa)
IF (Immunofluorescence)
(Immunofluorescent analysis of A549 cells using AAA26929 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of A549 cells using AAA26929 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human melanoma using AAA26929 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human melanoma using AAA26929 at dilution of 1:100)
Related Product Information for anti-CA1 antibody
Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.
Product Categories/Family for anti-CA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
carbonic anhydrase 1 isoform a
NCBI Official Synonym Full Names
carbonic anhydrase I
NCBI Official Symbol
CA1
NCBI Official Synonym Symbols
CAB; CA-I; Car1; HEL-S-11
NCBI Protein Information
carbonic anhydrase 1
UniProt Protein Name
Carbonic anhydrase 1
UniProt Gene Name
CA1
UniProt Synonym Gene Names
CAB; CA-I
UniProt Entry Name
CAH1_HUMAN
Similar Products
Product Notes
The CA1 ca1 (Catalog #AAA26929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Carbonic anhydrase 1 polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic anhydrase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP) . Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 . Researchers should empirically determine the suitability of the CA1 ca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic anhydrase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.