IF (Immunofluorescence)
(AAA328881 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328881 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit Carbonic Anhydrase IX Polyclonal Antibody | anti-CA9 antibody
Carbonic Anhydrase IX Antibody
Gene Names
CA9; MN; CAIX
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Applications
ELISA, Immunocytochemistry, Immunofluorescence
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Carbonic Anhydrase IX, Antibody; Carbonic Anhydrase IX Antibody; CA-IX; CA9; CAH9_HUMAN; CAIX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; Carbonic dehydratase; G250; Membrane antigen MN; MN; P54/58N; pMW1; RCC associated protein G250; RCC-associated antigen G250; Renal cell carcinoma-associated antigen G250; anti-CA9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
Carbonic Anhydrase IX Antibody detects endogenous levels of total Carbonic Anhydrase IX.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
459
Applicable Applications for anti-CA9 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
A synthesized peptide derived from human Carbonic Anhydrase IX, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328881 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328881 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328881 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328881 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IHC (Immunohiostchemistry)
(AAA328881 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328881 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from EC304 cells, using Carbonic Anhydrase IX Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from EC304 cells, using Carbonic Anhydrase IX Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-CA9 antibody
Function: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Post Translational Modifications: Asn-346 bears high-mannose type glycan structures.
Subcellular Location: Nucleus. Nucleus>Nucleolus. Cell membrane>Single-pass type I membrane protein. Cell projection>Microvillus membrane>Single-pass type I membrane protein. Note: Found on the surface microvilli and in the nucleus, particularly in nucleolus.
Tissue Specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Subunit Structure: Forms oligomers linked by disulfide bonds.
Similarity: Belongs to the alpha-carbonic anhydrase family.
Post Translational Modifications: Asn-346 bears high-mannose type glycan structures.
Subcellular Location: Nucleus. Nucleus>Nucleolus. Cell membrane>Single-pass type I membrane protein. Cell projection>Microvillus membrane>Single-pass type I membrane protein. Note: Found on the surface microvilli and in the nucleus, particularly in nucleolus.
Tissue Specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Subunit Structure: Forms oligomers linked by disulfide bonds.
Similarity: Belongs to the alpha-carbonic anhydrase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)49kD.
Predicted Molecular Weight: (Calculated)50kDa.
Predicted Molecular Weight: (Calculated)50kDa.
NCBI Official Full Name
carbonic anhydrase IX
NCBI Official Synonym Full Names
carbonic anhydrase 9
NCBI Official Symbol
CA9
NCBI Official Synonym Symbols
MN; CAIX
NCBI Protein Information
carbonic anhydrase 9
UniProt Protein Name
Carbonic anhydrase 9
UniProt Gene Name
CA9
UniProt Synonym Gene Names
G250; MN; CA-IX; CAIX; RCC-associated antigen G250
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Product Notes
The CA9 ca9 (Catalog #AAA328881) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Carbonic Anhydrase IX Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase IX can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the CA9 ca9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase IX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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