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WB (Western Blot)
(Figure 1. Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (AAA45178).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat heart tissue lysate,Lane 2: mouse heart tissue lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15KD. The expected band size for Cardiac FABP is at 15KD.)
Rabbit anti-Human Cardiac FABP Polyclonal Antibody | anti-FABP3 antibody
Anti-Cardiac FABP antibody
Gene Names
FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Cardiac FABP, Antibody; Anti-Cardiac FABP antibody; Fatty acid-binding protein, heart; fatty acid binding protein 3, muscle and heart(mammary-derived growth inhibitor); 422 protein antibody; Cardiac Fatty Acid Binding Protein antibody; FABP 11 antibody; FABP 3 antibody; FABP11 antibody; FABP3 antibody; FABPH_HUMAN antibody; Fatty acid binding protein 11 antibody; Fatty acid binding protein 3 antibody; Fatty acid binding protein 3 muscle and heart antibody; Fatty acid binding protein 3 muscle and heart mammary derived growth inhibitor antibody; Fatty acid binding protein 3 muscle antibody; Fatty acid binding protein 3, muscle and heart(mammary derived growth inhibitor) antibody; Fatty acid binding protein 3, muscle antibody; Fatty acid binding protein heart antibody; Fatty acid binding protein, heart antibody; Fatty acid binding protein, muscle and heart antibody; Fatty acid binding protein, skeletal muscle antibody; Fatty acid-binding protein 3 antibody; Fatty acid-binding protein antibody; H FABP antibody; H-FABP antibody; heart antibody; Heart type fatty acid binding protein antibody; Heart-type fatty acid-binding protein antibody; M FABP antibody; M-FABP antibody; Mammary derived growth inhibitor antibody; Mammary-derived growth inhibitor antibody; MDGI antibody; Muscle fatty acid binding protein antibody; Muscle fatty acid-binding protein antibody; Mylein protein P2 homolog antibody; O FABP antibody; OTTHUMP00000003898 antibody; P2 adopocyte protein antibody; anti-FABP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
133
Applicable Applications for anti-FABP3 antibody
IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cardiac FABP (119-133aa THGTAVCTRTYEKEA).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 1. Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (AAA45178).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat heart tissue lysate,Lane 2: mouse heart tissue lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15KD. The expected band size for Cardiac FABP is at 15KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (AAA45178).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat heart tissue lysate,Lane 2: mouse heart tissue lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15KD. The expected band size for Cardiac FABP is at 15KD.)
IHC (Immunohistochemisry)
(Cardiac FABP was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- Cardiac FABP Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohistochemisry)
(Cardiac FABP was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- Cardiac FABP Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Cardiac FABP was detected in paraffin-embedded sections of mouse skeletal muscletissues using rabbit anti- Cardiac FABP Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Cardiac FABP was detected in paraffin-embedded sections of mouse skeletal muscletissues using rabbit anti- Cardiac FABP Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohistochemistry)
(Cardiac FABP was detected in paraffin-embedded sections of rat cardiac muscle tissues using rabbit anti- Cardiac FABP Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohistochemistry)
(Cardiac FABP was detected in paraffin-embedded sections of rat cardiac muscle tissues using rabbit anti- Cardiac FABP Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
Related Product Information for anti-FABP3 antibody
Description: Rabbit IgG polyclonal antibody for Fatty acid-binding protein, heart(FABP3) detection. Tested with WB, IHC-P, IHC-F in Human.
Background: Heart-type fatty acid binding protein(hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins(FABPs) belongs to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein(cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.
Background: Heart-type fatty acid binding protein(hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins(FABPs) belongs to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein(cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14,858 Da
NCBI Official Full Name
Fatty acid-binding protein, heart
NCBI Official Synonym Full Names
fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
NCBI Official Symbol
FABP3
NCBI Official Synonym Symbols
MDGI; FABP11; H-FABP; M-FABP; O-FABP
NCBI Protein Information
fatty acid-binding protein, heart; fatty acid binding protein 11; mammary-derived growth inhibitor; muscle fatty acid-binding protein; Fatty acid-binding protein 3, muscle; heart-type fatty acid-binding protein
UniProt Protein Name
Fatty acid-binding protein, heart
UniProt Gene Name
FABP3
UniProt Synonym Gene Names
FABP11; MDGI; H-FABP; MDGI; M-FABP
UniProt Entry Name
FABPH_HUMAN
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Product Notes
The FABP3 fabp3 (Catalog #AAA45178) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cardiac FABP antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cardiac FABP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the FABP3 fabp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cardiac FABP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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