WB (Western Blot)
(Detection of human CARMIL by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARMIL antibody AAA212769 (lot AAA212769-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human CARMIL Polyclonal Antibody | anti-CARMIL antibody
Rabbit anti-CARMIL Antibody, Affinity Purified
Gene Names
LRRC16A; CARMIL; LRRC16; CARMIL1; CARMIL1a; dJ501N12.1; dJ501N12.5
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CARMIL, Antibody; Rabbit anti-CARMIL Antibody, Affinity Purified; CARMIL homolog; LRRC16A; LRRC16; leucine-rich repeat-containing protein 16A; leucine rich repeat containing 16A; leucine rich repeat containing 16; testicular tissue protein Li 107; F-actin-uncapping protein LRRC16A; CARMIL1a; CARMIL; capping protein, Arp2/3, and Myosin-I Linker homolog 1; capping protein, Arp2/3 and myosin-I linker protein 1; capping protein, Arp2/3 and myosin-I linker homolog 1; capping protein regulator and myosin 1 linker protein 1; dJ501N12.1; dJ501N12.5; capping protein regulator and myosin 1 linker 1; anti-CARMIL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CARMIL antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 1321 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CARMIL by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARMIL antibody AAA212769 (lot AAA212769-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human CARMIL by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARMIL antibody AAA212769 (lot AAA212769-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CARMIL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARMIL antibody AAA212769 (lot AAA212769-1) used for IP at 6 ug per reaction. CARMIL was also immunoprecipitated by rabbit anti-CARMIL antibody BL17273. For blotting immunoprecipitated CARMIL, AAA212769 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CARMIL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARMIL antibody AAA212769 (lot AAA212769-1) used for IP at 6 ug per reaction. CARMIL was also immunoprecipitated by rabbit anti-CARMIL antibody BL17273. For blotting immunoprecipitated CARMIL, AAA212769 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-CARMIL antibody
CARMIL binds CAPZA2 with high affinity and significantly decreases CAPZA2 affinity for actin barbed ends. It increases the rate of elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. CARMIL Rapidly uncaps barbed ends capped by CAPZA2 and enhances barbed-end actin polymerization (By similarity).CARMIL may control actin dynamics in lamellipodi and be required for cell migration [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5VZK9].
Product Categories/Family for anti-CARMIL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
151,557 Da
NCBI Official Full Name
leucine-rich repeat-containing protein 16A isoform 1
NCBI Official Synonym Full Names
leucine rich repeat containing 16A
NCBI Official Symbol
LRRC16A
NCBI Official Synonym Symbols
CARMIL; LRRC16; CARMIL1; CARMIL1a; dJ501N12.1; dJ501N12.5
NCBI Protein Information
leucine-rich repeat-containing protein 16A; CARMIL homolog; leucine rich repeat containing 16; capping protein, Arp2/3, and Myosin-I Linker homolog 1
UniProt Protein Name
Leucine-rich repeat-containing protein 16A
UniProt Gene Name
LRRC16A
UniProt Synonym Gene Names
CARMIL; CARMIL1a; LRRC16
UniProt Entry Name
LR16A_HUMAN
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Product Notes
The CARMIL lrrc16a (Catalog #AAA212769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CARMIL Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CARMIL can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CARMIL lrrc16a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARMIL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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