WB (Western Blot)
(Detection of human and mouse CARMIL2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, MCF-7, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARMIL2 antibody (AAA213445 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse CARMIL2 Polyclonal Antibody | anti-CARMIL2 antibody
Rabbit anti-CARMIL2 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CARMIL2, Antibody; Rabbit anti-CARMIL2 Antibody, Affinity Purified; F-actin-uncapping protein RLTPR; RGD, leucine-rich repeat, tropomodulin and proline-rich-containing protein; RGD, leucine-rich repeat, tropomodulin and proline-rich containing protein; RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; LRRC16C; leucine-rich repeat-containing protein 16C; IMD58; RLTPR; CARMIL2b; capping protein, Arp2/3 and myosin-I linker protein 2; Capping protein regulator and myosin 1 linker 2; leucine rich repeat containing 16C; capping protein regulator and myosin 1 linker 2; anti-CARMIL2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CARMIL2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1385 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse CARMIL2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, MCF-7, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARMIL2 antibody (AAA213445 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse CARMIL2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, MCF-7, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARMIL2 antibody (AAA213445 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CARMIL2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARMIL2 antibody (AAA213445 lot 1) used for IP at 1 ug per reaction. CARMIL2 was also immunoprecipitated by rabbit anti-CARMIL2 antibody For blotting immunoprecipitated CARMIL2, AAA213445 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CARMIL2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARMIL2 antibody (AAA213445 lot 1) used for IP at 1 ug per reaction. CARMIL2 was also immunoprecipitated by rabbit anti-CARMIL2 antibody For blotting immunoprecipitated CARMIL2, AAA213445 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-CARMIL2 antibody
Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization. Plays a role in cell protrusion formations; involved in cell polarity, lamellipodial assembly, membrane ruffling and macropinosome formations. Involved as well in cell migration and invadopodia formation during wound healing. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6F5E8].
Product Categories/Family for anti-CARMIL2 antibody
NCBI and Uniprot Product Information
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Product Notes
The CARMIL2 rltpr (Catalog #AAA213445) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CARMIL2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CARMIL2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CARMIL2 rltpr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARMIL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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