Rabbit CASP1 Polyclonal Antibody | anti-CASP1 antibody

CASP1 Antibody

Cat. Num. AAA325930

• Gene Names: CASP1; ICE; P45; IL1BC
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CASP1, Antibody; CASP1 Antibody; CASP-1; CASP1; CASP1_HUMAN; Caspase 1; Caspase-1 subunit p10; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1 beta converting enzyme; IL1B convertase; Interleukin 1 beta convertase; Interleukin 1B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45; anti-CASP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CASP1 antibody detects endogenous levels of total CASP1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 383

• Applicable Applications for anti-CASP1 antibody: ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human CASP1
• Subcellular Location: Cytoplasm.
• Tissue Specificity: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemisry)

(AAA325930 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence)

(AAA325930 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot)

(Western blot analysis of Mouse Spleen tissue lysate, using CASP1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-CASP1 antibody

Description: Caspase-1, or interleukin-1 converting enzyme (ICE/ICEalpha), is a class I cysteine protease, which also includes caspases-4, -5, -11, and-12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1 and interferon-gamma inducing factor (IL-18) into their mature forms (1, 2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1beta and are resistant to endotoxic shock (4, 5). At least six caspase-1 isoforms have been identified, including caspase-1 alpha, beta, gamma, delta, epsilon and zeta (6). Most caspase-1 isoforms (alpha, beta, gamma and delta) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 epsilon typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative. The widely expressed zeta isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the alpha isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the inflammasome that includes caspase-5, Pycard/Asc, and NALP1 (7).
Function: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).
Subunit Structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p10 and p20 subunits interact with MEFV. Interacts with CARD17/INCA and CARD18.
Post-translational Modifications: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Similarity: Belongs to the peptidase C14A family.

NCBI and Uniprot Product Information

• NCBI GI #: 4502573
• NCBI GeneID: 834
• NCBI Accession #: NP_001214.1
• NCBI GenBank Nucleotide #: NM_001223.4
• UniProt Accession #: P29466; Q53EY6; Q6DMQ1; Q6GSS3; Q6PI75; Q9UCN3; B5MDZ1
• Molecular Weight: Observed: 45 kDa; Predicted: 46 kDa
• NCBI Official Full Name: caspase-1 isoform beta
• NCBI Official Synonym Full Names: caspase 1
• NCBI Official Symbol: CASP1
• NCBI Official Synonym Symbols: ICE; P45; IL1BC
• NCBI Protein Information: caspase-1
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: CASP1
• UniProt Synonym Gene Names: IL1BC; IL1BCE; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme