IP (Immunoprecipitation)
(CASP8 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-CASP8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CASP8 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :55 kDa)
Rabbit anti-Human CASP8/ALPS2B Polyclonal Antibody | anti-CASP8/ALPS2B antibody
Anti-CASP8/ALPS2B Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CASP8/ALPS2B, Antibody; Anti-CASP8/ALPS2B Antibody, Rabbit Polyclonal; caspase 8; apoptosis-related cysteine peptidase; Anti-ALPS2B Antibody; Anti-CAP4 Antibody; Anti-Casp-8 Antibody; Anti-FLICE Antibody; Anti-MACH Antibody; Anti-MCH5 Antibody; anti-CASP8/ALPS2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CASP8/ALPS2B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-CASP8/ALPS2B antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
E coli-derived Human CASP8/ALPS2B fragment
Preparation
Produced in rabbits immunized with E coli-derived Human CASP8/ALPS2B fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(CASP8 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-CASP8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CASP8 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :55 kDa)
IP (Immunoprecipitation)
(CASP8 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-CASP8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CASP8 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :55 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human CASP8 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human CASP8 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-CASP8 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa)
WB (Western Blot)
(Anti-CASP8 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa)
Related Product Information for anti-CASP8/ALPS2B antibody
Caspase 8 is a cysteine dependent endoprotease that plays a key role in the regulation of apoptosis (also referred as programmed cell death), an event which is known to be essential in growth and development of embryo, and maintenance of tissue homeostasis. Caspases are broadly classified as apoptosis or inflammation regulatory caspases based on their role in respective processes. Of those known, caspase-3, -6, -7, -8 and -9 are categorized as apoptotic caspases, whereas caspase -1, -4, -5 and -12 are grouped as inflammatory caspases. The apoptotic caspases are further classified into initiator caspases (caspase -8 and -9) and effecter caspases (caspase -3, -6 and -7) based on their role in transducing the apoptotic signals emanating at the cell membrane.
References
Menon U, Raghuram P H, Kannan K, et al. Mutation Analysis of the Dimer Forming Domain of the Caspase 8 Gene in Oral Submucous Fibrosis and Squamous Cell Carcinomas[J]. Asian Pacific journal of cancer prevention: APJCP, 2014, 16(11): 4589-4592.
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Product Notes
The CASP8/ALPS2B (Catalog #AAA259027) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CASP8/ALPS2B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CASP8/ALPS2B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CASP8/ALPS2B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CASP8/ALPS2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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