Rabbit Caspase-3 Polyclonal Antibody | anti-Casp3 antibody

Polyclonal Antibody to Caspase-3 (Pro and Active)

Cat. Num. AAA78323

• Gene Names: Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Caspase-3; A830040C14Rik
• Reactivity: Dog, Gerbil, Human, Mouse, Rat
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Serum
• Synonyms: Caspase-3, Antibody; Polyclonal Antibody to Caspase-3 (Pro and Active); Casp3; Cpp32; Apopain; CPP-32; Cysteine protease CPP32; LICE; Protein Yama; SCA-1; SREBP cleavage activity 1; Antibody to Caspase-3 (Pro and Active); Anti-Caspase-3 (Pro and Active) Antibody; Caspase-3 (Pro and Active) Antibody; anti-Casp3 antibody

• Host: Rabbit
• Reactivity: Dog, Gerbil, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Serum
• Form/Format: 50 ul sera
• Sequence Length: 277

• Applicable Applications for anti-Casp3 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Information: A full-length recombinant protein of human Caspase-3 (pro-form) was used as immunogen for this antibody
• Antigen: Caspase-3 (Pro and Active)
• Preparation and Storage: Store the antibody at 4 degree C, stable for 6 months.; For long-term storage, store at -20 degree C.; Avoid repeated freeze and thaw cycles.

IHC (Immunohistochemisry)

(Fig: 3 Immunohistochemical analysis of Caspase-3 expression in formalin-fixed, paraffin-embedded human breast ductal carcinoma in situ using at 1: 2000. Staining is seen in the the cancerous ducts, but not in the normal lobulus.)

IHC (Immunohiostchemistry)

(Fig: 2 Immunohistochemical analysis of Caspase-3 expression in formalin-fixed, paraffin-embedded human reactive lymph node using at 1: 2000. Staining is seen in the apoptosis-prone germinal center B lymphocytes of follicles. In constrast little or no staining is seen in the surrounding long-live mantle zone lymphocytes.)

WB (Western Blot)

(Fig: 1 Western blot analysis of Caspase-3. Lysates from Jurkat cells (lane 1), normal mammary tissue (lane 2) and surgical specimens from three invasive ductal carcinomas (lanes 3-5)were normalized for total protein content (50 ug/lane) and Fig: 1 Western blotted with anti-Caspase-3. The ~32 kDa pro-Caspase-3 protein was detected in all samples. Active/cleaved Caspase-3 was identified in Jurkat (10 kDa small subunit, lane 1) and two ductal carcinomas (14-21 kDa large subunit).)

Related Product Information for anti-Casp3 antibody

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Caspase-3 polyclonal antisera recognizes the proform of caspase-3 (approx. 32) kDa), and the large (approx. 14-21 kDa) and small (approx. 10 kDa) subunits of active/cleaved Caspase-3.

Product Categories/Family for anti-Casp3 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 2493527
• NCBI GeneID: 12367
• UniProt Accession #: P70677; O08668; Q8CHV5; Q9QWI4
• Molecular Weight: 31,475 Da
• NCBI Official Full Name: Caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: Casp3
• NCBI Official Synonym Symbols: CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Caspase-3; A830040C14Rik
• NCBI Protein Information: caspase-3
• UniProt Protein Name: Caspase-3
• UniProt Gene Name: Casp3
• UniProt Synonym Gene Names: Cpp32; CASP-3; CPP-32; SCA-1