IHC (Immunohistochemisry)
(Anti-Caspase-3(P10), AAA45466, IHC(P)IHC(P): Human Lung Cancer Tissue)
Rabbit anti-Human Caspase-3(P10) Polyclonal Antibody | anti-CASP3 antibody
Anti-Caspase-3(P10)
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Caspase-3(P10), Antibody; Anti-Caspase-3(P10); Caspase-3(CASP-3); caspase 3, apoptosis-related cysteine peptidase; A830040C14Rik antibody; Apopain antibody; Apopain precursor antibody; CASP 3 antibody; CASP-3 antibody; CASP3 antibody; Casp3a antibody; Caspase 3 antibody; Caspase 3 apoptosis related cysteine protease antibody; Caspase 3 p12 subunit antibody; Caspase 3, apoptosis-related cysteine peptidase antibody; Caspase 3, apoptosis-related cysteine protease antibody; Caspase 3, apoptosis-related cysteine protease a antibody; Caspase-3 subunit p12 antibody; Caspase3 antibody; CC3 antibody; CPP 32 antibody; CPP-32 antibody; CPP32 antibody; CPP32B antibody; Cysteine protease CPP32 antibody; EC 3.4.22.56 antibody; Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody; ICE3 antibody; LICE antibody; mldy antibody; OTTHUMP00000165052 antibody; OTTHUMP00000165053 antibody; OTTHUMP00000165054 antibody; PARP cleavage protease antibody; Procaspase3 antibody; Protein Yama antibody; SCA 1 antibody; SCA-1 antibody; SCA1 antibody; SREBP cleavage activity 1 antibody; Yama antibody; Yama protein antibody; anti-CASP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
277
Applicable Applications for anti-CASP3 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-3 (P10) (220-236aa CAMLKQYADKLEFMHIL).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(Anti-Caspase-3(P10), AAA45466, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohistochemisry)
(Anti-Caspase-3(P10), AAA45466, IHC(P)IHC(P): Human Lung Cancer Tissue)
WB (Western Blot)
(Anti-Caspase-3(P10), AAA45466, Western blottingLane 1: HELA Cell LysateLane 2: SMMC Cell Lysate)
WB (Western Blot)
(Anti-Caspase-3(P10), AAA45466, Western blottingLane 1: HELA Cell LysateLane 2: SMMC Cell Lysate)
IHC (Immunohistochemistry)
(Anti-Caspase-3(P10), AAA45466, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-Caspase-3(P10), AAA45466, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
Related Product Information for anti-CASP3 antibody
Description: Rabbit IgG polyclonal antibody for Caspase-3(CASP3) detection. Tested with WB, IHC-P, ICC in Human.
Background: Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located at 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9; and the protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.
Background: Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located at 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9; and the protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31,608 Da
NCBI Official Full Name
Caspase-3
NCBI Official Synonym Full Names
caspase 3, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP3
NCBI Official Synonym Symbols
CPP32; SCA-1; CPP32B
NCBI Protein Information
caspase-3; CASP-3; CPP-32; apopain; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease
UniProt Protein Name
Caspase-3
UniProt Gene Name
CASP3
UniProt Synonym Gene Names
CPP32; CASP-3; CPP-32; SCA-1
UniProt Entry Name
CASP3_HUMAN
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Product Notes
The CASP3 casp3 (Catalog #AAA45466) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Caspase-3(P10) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-3(P10) can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CASP3 casp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-3(P10), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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