IHC (Immunohistochemisry)
(Fig: 3 Formalin-fixed paraffin-embedded tissue section of dog cerebellum stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1: 2000. Hematoxylin-eosin counterstain.)
Rabbit Caspase-6 Polyclonal Antibody | anti-Casp6 antibody
Polyclonal Antibody to Caspase-6
Reactivity
Dog, Rat, Mouse, Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Serum
Synonyms
Caspase-6, Antibody; Polyclonal Antibody to Caspase-6; Casp6; LOC103689977; rCG_28913; Antibody to Caspase-6; Anti-Caspase-6 Antibody; Caspase-6 Antibody; anti-Casp6 antibody
Host
Rabbit
Reactivity
Dog, Rat, Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum
Form/Format
50 ul sera
Sequence Length
277
Applicable Applications for anti-Casp6 antibody
WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen Information
A recombinant catalytically active human Caspase-6 protein was used as the immunogen for this antibody
Antigen
Caspase-6
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
IHC (Immunohistochemisry)
(Fig: 3 Formalin-fixed paraffin-embedded tissue section of dog cerebellum stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1: 2000. Hematoxylin-eosin counterstain.)
IHC (Immunohistochemisry)
(Fig: 3 Formalin-fixed paraffin-embedded tissue section of dog cerebellum stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1: 2000. Hematoxylin-eosin counterstain.)
IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed paraffin-embedded tissue sections of human gliomas stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1: 2000. Gemistocytoma (low grade tumor) at low (A) and high (B) magnification. Two examples of anaplastic, high grade (III) infiltrating gliomas (C and D). Increased active/cleaved Caspase-6 may be seen in high grade, compared to low grade tumors. Hematoxylin-eosin counterstain.)
IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed paraffin-embedded tissue sections of human gliomas stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1: 2000. Gemistocytoma (low grade tumor) at low (A) and high (B) magnification. Two examples of anaplastic, high grade (III) infiltrating gliomas (C and D). Increased active/cleaved Caspase-6 may be seen in high grade, compared to low grade tumors. Hematoxylin-eosin counterstain.)
WB (Western Blot)
(Fig: 1 Western blot analysis of Caspase-6 using Caspase-6 polyclonal antibody. Recombinant catalytically active caspase-6 was Fig: 1 Western blotted with Active/Cleaved Caspase-6 antibody. The antibody detected both the large and small subunits of Caspase-6.)
WB (Western Blot)
(Fig: 1 Western blot analysis of Caspase-6 using Caspase-6 polyclonal antibody. Recombinant catalytically active caspase-6 was Fig: 1 Western blotted with Active/Cleaved Caspase-6 antibody. The antibody detected both the large and small subunits of Caspase-6.)
Related Product Information for anti-Casp6 antibody
Caspases belong to the cysteine-aspartic acid protease (Caspase) family which plays a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream. Caspase 3 (CPP32) and Caspase 6 (Mch2) are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis-inducing stimuli and in all cell lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Caspase-6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.This Active/Cleaved Caspase-6 polyclonal antibody recognizes the large and small subunits of caspase-6. Whereas the antisera has a strong preference for active/cleaved caspase-6, in some cell or tissue systems or techniques the antisera may also recognize the ~34 kDa proform of caspase-6 as well as intermediate caspase-6 cleavage fragments.
Product Categories/Family for anti-Casp6 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Casp6 casp6 (Catalog #AAA78324) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Caspase-6 reacts with Dog, Rat, Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-6 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the Casp6 casp6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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