IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed, paraffin-embedded sections from a brain tissue array stained for Caspase-8 expression using AAA78320 at 1: 2000. A. Normal brain stem (1) and cortex (2). B. Higher magnification of cortex (from A). C. Higher magnification of brain stem (from A). D. Normal cerebellum showing caspase-8 staining in the Purkinge cells.)
Rabbit Caspase 8 Polyclonal Antibody | anti-Casp8 antibody
Polyclonal Antibody to Caspase 8 (active/cleaved)
Gene Names
Casp8; MACH; Mch5; FLICE; CASP-8
Reactivity
Dog, Gerbil, Human, Mouse, Rat
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Synonyms
Caspase 8, Antibody; Polyclonal Antibody to Caspase 8 (active/cleaved); Casp8; CASP-8; Antibody to Caspase 8 (active/cleaved); Anti-Caspase 8 (active/cleaved) Antibody; Caspase 8 (active/cleaved) Antibody; anti-Casp8 antibody
Host
Rabbit
Reactivity
Dog, Gerbil, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Form/Format
50 ul sera
Sequence Length
480
Applicable Applications for anti-Casp8 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Information
A recombinant catalytically active of human Caspase-8 (active/cleaved) protein was used as the immunogen for this antibody
Preparation and Storage
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed, paraffin-embedded sections from a brain tissue array stained for Caspase-8 expression using AAA78320 at 1: 2000. A. Normal brain stem (1) and cortex (2). B. Higher magnification of cortex (from A). C. Higher magnification of brain stem (from A). D. Normal cerebellum showing caspase-8 staining in the Purkinge cells.)
IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed, paraffin-embedded sections from a brain tissue array stained for Caspase-8 expression using AAA78320 at 1: 2000. A. Normal brain stem (1) and cortex (2). B. Higher magnification of cortex (from A). C. Higher magnification of brain stem (from A). D. Normal cerebellum showing caspase-8 staining in the Purkinge cells.)
IHC (Immunohistochemistry)
(Fig: 1 Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using AAA78320 at 1: 2000. A. Anaplastic glioma (Grade III, left) and Gemistocytoma (Grade II, right) cores showing negative and positive staining for Caspase-8, respectively. B. Higher magnification of the Gemistocytoma tumor (from A).)
IHC (Immunohistochemistry)
(Fig: 1 Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using AAA78320 at 1: 2000. A. Anaplastic glioma (Grade III, left) and Gemistocytoma (Grade II, right) cores showing negative and positive staining for Caspase-8, respectively. B. Higher magnification of the Gemistocytoma tumor (from A).)
Related Product Information for anti-Casp8 antibody
Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases. Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18. The Active/Cleaved Caspase-8 polyclonal antisera recognizes the large and small subunits of active/cleaved caspase-8. Whereas the antisera has a strong preference for active/cleaved caspase-8, in some cell or tissue systems or techniques the antisera may also recognize the proform of caspase-8 as well as intermediate caspase-8 cleavage fragments.
Product Categories/Family for anti-Casp8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Caspase-8
NCBI Official Synonym Full Names
caspase 8
NCBI Official Symbol
Casp8
NCBI Official Synonym Symbols
MACH; Mch5; FLICE; CASP-8
NCBI Protein Information
caspase-8
UniProt Protein Name
Caspase-8
UniProt Gene Name
Casp8
UniProt Synonym Gene Names
CASP-8
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Product Notes
The Casp8 casp8 (Catalog #AAA78320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Caspase 8 (active/cleaved) reacts with Dog, Gerbil, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase 8 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Casp8 casp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase 8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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