Rabbit Caspase-9 Polyclonal Antibody | anti-CASP9 antibody

Polyclonal Antibody to Caspase-9

Cat. Num. AAA78326

• Gene Names: CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• Reactivity: Dog, Rat, Mouse, Human
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Serum
• Synonyms: Caspase-9, Antibody; Polyclonal Antibody to Caspase-9; CASP9; MCH6; anti-CASP9 antibody

• Host: Rabbit
• Reactivity: Dog, Rat, Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Serum
• Form/Format: Sera
• Sequence Length: 416

• Applicable Applications for anti-CASP9 antibody: IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Information: A full-length recombinant protein of human Caspase-9 was used as the immunogen for this antibody
• Antigen: Caspase-9
• Preparation and Storage: Store the antibody at 4 degree C, stable for 6 months.; For long-term storage, store at -20 degree C.; Avoid repeated freeze and thaw cycles.

IHC (Immunohiostchemistry)

(Fig: 2 Immunohistochemical analysis of Caspase-9 expression in formalin-fixed, paraffin-embedded mouse brain tissue sections using at 1: 2000. Hematoxylin-eosin counterstain. A: Brain striatum. B: Brain stem motor neuron.)

WB (Western Blot)

(Fig: 1 Western blot analysis of Caspase-9. Various tissue lysates were prepared from human autospsy material and normalized for total protein. Most tissues contained the ~50 kDa pro-Caspase-9 protein. Cleaved Caspase-9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9: recombinant human pro-Caspase-9 (full-length) protein. PRO: pro-Caspase-9. L: large subunit of cleaved Caspase-9. S: small subunit of cleaved Caspase-9.)

Related Product Information for anti-CASP9 antibody

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Caspase-9 polyclonal antisera recognizes the pro form of caspase-9 (approx. 50 kDa), and the large (approx. 35 kDa) and small (approx. 15 kDa) subunits of active/cleaved Caspase-9.

Product Categories/Family for anti-CASP9 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 28558771
• NCBI GeneID: 842
• UniProt Accession #: P55211; O95348; Q53Y70; Q5JRU9; Q5UGI1; Q92852; Q9BQ62; Q9UEQ3; Q9UIJ8; B4E1A3
• NCBI Official Full Name: Caspase-9
• NCBI Official Synonym Full Names: caspase 9
• NCBI Official Symbol: CASP9
• NCBI Official Synonym Symbols: MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• NCBI Protein Information: caspase-9
• UniProt Protein Name: Caspase-9
• UniProt Gene Name: CASP9
• UniProt Synonym Gene Names: MCH6; CASP-9; APAF-3; ICE-LAP6