IP (Immunoprecipitation)
(CASP9 was immunoprecipitated using:Lane A:0.5 mg NIH-3T3 Whole Cell Lysate4 uL anti-CASP9 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CASP9 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size: 46 kDa)
Rabbit anti-Human Caspase-9 Polyclonal Antibody | anti-CASP9 antibody
Anti-Caspase-9 Antibody, Rabbit Polyclonal
Gene Names
CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Caspase-9, Antibody; Anti-Caspase-9 Antibody, Rabbit Polyclonal; Caspase-9 Antibody, Rabbit PAb, Antigen Affinity Purified; caspase 9, apoptosis-related cysteine peptidase; Anti-APAF-3 Antibody; Anti-APAF3 Antibody; Anti-ICE-LAP6 Antibody; Anti-MCH6 Antibody; Anti-PPP1R56 Antibody; anti-CASP9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Caspase-9
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CASP9 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human Caspase-9
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Caspase-9, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(CASP9 was immunoprecipitated using:Lane A:0.5 mg NIH-3T3 Whole Cell Lysate4 uL anti-CASP9 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CASP9 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size: 46 kDa)
IP (Immunoprecipitation)
(CASP9 was immunoprecipitated using:Lane A:0.5 mg NIH-3T3 Whole Cell Lysate4 uL anti-CASP9 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CASP9 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size: 46 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of CASP9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CASP9 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of CASP9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CASP9 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
WB (Western Blot)
(Anti-CASP9 rabbit polyclonal antibody at 1:500 dilutionLane A: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:55 kDa)
WB (Western Blot)
(Anti-CASP9 rabbit polyclonal antibody at 1:500 dilutionLane A: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:55 kDa)
Related Product Information for anti-CASP9 antibody
Neural tube defects (NTDs) are birth defects of the nervous system and are the second most frequent cause of birth defects worldwide. The etiology of NTDs is complicated and involves both genetic and environmental factors. CASP9 is an initiator caspase in the intrinsic apoptosis pathway, which in Casp9-/-mice has been shown to result in NTDs because of decreased apoptosis. HSP90 inhibitors are identified here as a pharmacological approach for targeting autophagy via destabilization of ATG7, while an induced expression of CASP9, but not its apoptotic activity, is identified as a resistance mechanism to the cellular stress brought about by HSP90 inhibition.
Product Categories/Family for anti-CASP9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,281 Da
NCBI Official Full Name
caspase-9 isoform alpha
NCBI Official Synonym Full Names
caspase 9, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP9
NCBI Official Synonym Symbols
MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
NCBI Protein Information
caspase-9; apoptotic protease MCH-6; ICE-like apoptotic protease 6; apoptotic protease activating factor 3; protein phosphatase 1, regulatory subunit 56
UniProt Protein Name
Caspase-9
UniProt Gene Name
CASP9
UniProt Synonym Gene Names
MCH6; CASP-9; APAF-3; ICE-LAP6
UniProt Entry Name
CASP9_HUMAN
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Product Notes
The CASP9 casp9 (Catalog #AAA254720) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Caspase-9 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-9 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CASP9 casp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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