FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-Caspase-9 p35/Casp9 antibody (AAA125515).Overlay histogram showing mouse spleen tissues stained with AAA125515 (Blue line). The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9 p35/Casp9 Antibody (AAA125515, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Mouse, Rat Caspase-9 p35/Casp9 Polyclonal Antibody | anti-Casp9 antibody
Anti-Caspase-9 p35/Casp9 Antibody
Gene Names
Casp9; Mch6; APAF-3; CASP-9; AI115399; AW493809; ICE-LAP6; Caspase-9
Reactivity
Mouse, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Caspase-9 p35/Casp9, Antibody; Anti-Caspase-9 p35/Casp9 Antibody; Casp9; Mch6; Caspase-9; CASP-9; EC 3. 4. 22. 62; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6; Caspase-9 subunit p35; caspase 9, apoptosis-related cysteine peptidase; anti-Casp9 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for Caspase-9 p35/Casp9 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-Casp9 antibody
ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E Coli-derived mouse Caspase-9 p35/Casp9 recombinant protein (Position: E3-D266).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-Caspase-9 p35/Casp9 antibody (AAA125515).Overlay histogram showing mouse spleen tissues stained with AAA125515 (Blue line). The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9 p35/Casp9 Antibody (AAA125515, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-Caspase-9 p35/Casp9 antibody (AAA125515).Overlay histogram showing mouse spleen tissues stained with AAA125515 (Blue line). The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9 p35/Casp9 Antibody (AAA125515, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of Caspase-9 p35/Casp9 using anti-Caspase-9 p35/Casp9 antibody (AAA125515).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: mouse heart tissue lysatesLane 2: rat heart tissue lysatesLane 3: rat skeletal muscle tissue lysates,After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Caspase-9 p35/Casp9 antigen affinity purified polyclonal antibody (Catalog # AAA125515) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Caspase-9 p35/Casp9 at approximately 37/46KD. The expected band size for Caspase-9 p35/Casp9 is at 37/46KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of Caspase-9 p35/Casp9 using anti-Caspase-9 p35/Casp9 antibody (AAA125515).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: mouse heart tissue lysatesLane 2: rat heart tissue lysatesLane 3: rat skeletal muscle tissue lysates,After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Caspase-9 p35/Casp9 antigen affinity purified polyclonal antibody (Catalog # AAA125515) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Caspase-9 p35/Casp9 at approximately 37/46KD. The expected band size for Caspase-9 p35/Casp9 is at 37/46KD.)
Related Product Information for anti-Casp9 antibody
Caspase-9 is an enzyme that in humans is encoded by the CASP9 gene. This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
References
1. Chew, S. K., Chen, P., Link, N., Galindo, K. A., Pogue, K., Abrams, J. M. Genome-wide silencing in Drosophila captures conserved apoptotic effectors. Nature 460: 123-127, 2009.
2. Hadano, S., Nasir, J., Nichol, K., Rasper, D. M., Vaillancourt, J. P., Sherer, S. W., Beatty, B. G., Ikeda, J. -E., Nicholson, D. W., Hayden, M. R. Genomic organization of the human caspase-9 gene on chromosome 1p36. 1-p36. 3. Mammalian Genome 10: 757-760, 1999.
3. Hakem, R., Hakem, A., Duncan, G. S., Henderson, J. T., Woo, M., Soengas, M. S., Elia, A., de la Pompa, J. L., Kagi, D., Khoo, W., Potter, J., Yoshida, R., Kaufman, S. A., Lowe, S. W., Penninger, J. M., Mak, T. W. Differential requirement for caspase 9 in apoptotic pathways in vivo. Cell 94: 339-352, 1998.
2. Hadano, S., Nasir, J., Nichol, K., Rasper, D. M., Vaillancourt, J. P., Sherer, S. W., Beatty, B. G., Ikeda, J. -E., Nicholson, D. W., Hayden, M. R. Genomic organization of the human caspase-9 gene on chromosome 1p36. 1-p36. 3. Mammalian Genome 10: 757-760, 1999.
3. Hakem, R., Hakem, A., Duncan, G. S., Henderson, J. T., Woo, M., Soengas, M. S., Elia, A., de la Pompa, J. L., Kagi, D., Khoo, W., Potter, J., Yoshida, R., Kaufman, S. A., Lowe, S. W., Penninger, J. M., Mak, T. W. Differential requirement for caspase 9 in apoptotic pathways in vivo. Cell 94: 339-352, 1998.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,979 Da
NCBI Official Full Name
caspase-9 isoform 1
NCBI Official Synonym Full Names
caspase 9
NCBI Official Symbol
Casp9
NCBI Official Synonym Symbols
Mch6; APAF-3; CASP-9; AI115399; AW493809; ICE-LAP6; Caspase-9
NCBI Protein Information
caspase-9
UniProt Protein Name
Caspase-9
UniProt Gene Name
Casp9
UniProt Synonym Gene Names
Mch6; CASP-9; APAF-3; ICE-LAP6
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Product Notes
The Casp9 casp9 (Catalog #AAA125515) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Caspase-9 p35/Casp9 Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-9 p35/Casp9 can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the Casp9 casp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-9 p35/Casp9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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