IF (Immunofluorescence)
(AAA329015 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329015 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit Caspr2 Polyclonal Antibody | anti-CNTNAP2 antibody
Caspr2 Antibody
Gene Names
CNTNAP2; CDFE; NRXN4; AUTS15; CASPR2; PTHSL1
Reactivity
Human, Mouse, RatPredicted Reactivity: Horse(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Caspr2, Antibody; Caspr2 Antibody; AUTS15; CDFE; Cell recognition molecule Caspr2; CNTNAP2; CNTP2; CNTP2_HUMAN; Contactin-associated protein-like 2; Homolog of Drosophila neurexin IV; NRXN4; PTHSL1; anti-CNTNAP2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(100%)
Predicted Reactivity: Horse(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
Caspr2 Antibody detects endogenous levels of total Caspr2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
1331
Applicable Applications for anti-CNTNAP2 antibody
ELISA
Immunogen
A synthesized peptide derived from human Caspr2, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA329015 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329015 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA329015 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329015 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using Caspr2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using Caspr2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from RAW264.7 cells, using Caspr2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from RAW264.7 cells, using Caspr2 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-CNTNAP2 antibody
Function: Required, with CNTNAP1, for radial and longitudinal organization of myelinated axons. Plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Demarcates the juxtaparanodal region of the axo-glial junction.
Subcellular Location: Membrane>Single-pass type I membrane protein. Cell projection>Axon. Cell junction>Paranodal septate junction. Note: Expressed in the juxtaparadonal region.
Tissue Specificity: Predominantly expressed in nervous system.
Subunit Structure: Interacts (via C-terminus) with KCNA2.
Similarity: Belongs to the neurexin family.
Subcellular Location: Membrane>Single-pass type I membrane protein. Cell projection>Axon. Cell junction>Paranodal septate junction. Note: Expressed in the juxtaparadonal region.
Tissue Specificity: Predominantly expressed in nervous system.
Subunit Structure: Interacts (via C-terminus) with KCNA2.
Similarity: Belongs to the neurexin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)148kD.
Predicted Molecular Weight: (Calculated)148kDa.
Predicted Molecular Weight: (Calculated)148kDa.
NCBI Official Full Name
contactin-associated protein-like 2
NCBI Official Synonym Full Names
contactin associated protein 2
NCBI Official Symbol
CNTNAP2
NCBI Official Synonym Symbols
CDFE; NRXN4; AUTS15; CASPR2; PTHSL1
NCBI Protein Information
contactin-associated protein-like 2
UniProt Protein Name
Contactin-associated protein-like 2
UniProt Gene Name
CNTNAP2
Similar Products
Product Notes
The CNTNAP2 cntnap2 (Catalog #AAA329015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspr2 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Horse(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's Caspr2 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CNTNAP2 cntnap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspr2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.