Rabbit anti-Mouse Cathepsin A Polyclonal Antibody | anti-CTSA antibody

Anti-Cathepsin A Antibody, Rabbit Polyclonal

Cat. Num. AAA257138

• Gene Names: Ctsa; PPCA; Ppgb; AU019505
• Reactivity: Mouse
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Cathepsin A, Antibody; Anti-Cathepsin A Antibody, Rabbit Polyclonal; Cathepsin A/CTSA Antibody, Rabbit PAb, Antigen Affinity Purified; cathepsin A; Anti-AU019505 Antibody; Anti-PPCA Antibody; Anti-Ppgb Antibody; anti-CTSA antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Cathepsin A/CTSA
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CTSA antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Immunogen: Recombinant Mouse Cathepsin A/CTSA protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse Cathepsin A/CTSA. Cathepsin A/CTSA specific IgG was purified by Mouse Cathepsin A/CTSA affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-CTSA rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:28 kDa)

IP (Immunoprecipitation)

(Mouse CTSA was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse CTSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CTSA rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 30 kDa)

Related Product Information for anti-CTSA antibody

Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.

Product Categories/Family for anti-CTSA antibody

Serine Proteases and Regulators

References

Hiraiwa M. (1999) Cathepsin A/protective protein: an unusual lysosomal multifunctional protein. Cell Mol Life Sci. 56(11-12): 894-907.Yoshida T, et al. (2006) Comparative analysis of binding energy of chymostatin with human cathepsin A and its homologous proteins by molecular orbital calculation. J Chem Inf Model. 46(5): 2093-103.Seyrantepe V, et al. (2008) Enzymatic activity of lysosomal carboxypeptidase (cathepsin) A is required for proper elastic fiber formation and inactivation of endothelin-1. Circulation. 117(15): 1973-81.

NCBI and Uniprot Product Information

• NCBI GI #: 84042523
• NCBI GeneID: 19025
• NCBI Accession #: NP_001033581.1
• NCBI GenBank Nucleotide #: NM_001038492.2
• UniProt Accession #: P16675; Q8VEF6
• Molecular Weight: 53,844 Da
• NCBI Official Full Name: lysosomal protective protein isoform b preproprotein
• NCBI Official Synonym Full Names: cathepsin A
• NCBI Official Symbol: Ctsa
• NCBI Official Synonym Symbols: PPCA; Ppgb; AU019505
• NCBI Protein Information: lysosomal protective protein
• UniProt Protein Name: Lysosomal protective protein
• UniProt Gene Name: Ctsa
• UniProt Synonym Gene Names: Ppgb; PPCA