Rabbit anti-Human Cathepsin B Polyclonal Antibody | anti-CTSB antibody

Anti-Cathepsin B Antibody, Rabbit Polyclonal

Cat. Num. AAA254958

• Gene Names: CTSB; APPS; CPSB
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Cathepsin B, Antibody; Anti-Cathepsin B Antibody, Rabbit Polyclonal; Cathepsin B Antibody, Rabbit PAb, Antigen Affinity Purified; cathepsin B; Anti-APPS Antibody; Anti-CPSB Antibody; anti-CTSB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Cathepsin B/CTSB
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CTSB antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant Human Cathepsin B/CTSB protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Cathepsin B/CTSB (rh Cathepsin B/CTSB). Cathepsin B/CTSB specific IgG was purified by Human Cathepsin B/CTSB affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(CTSB was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Raw264.7 Whole Cell LysateLane C:0.5 mg A549 Whole Cell Lysate2 uL anti-CTSB rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CTSB rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)

WB (Western Blot)

(Anti-CTSB rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: RAW264.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:38 kDaObserved band size:33 kDa(We are unsure as to the identity of these extra bands.))

IHC (Immunohiostchemistry)

(Immunochemical staining of human CTSB in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry)

(Immunochemical staining of human CTSB in human liver with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-CTSB antibody

Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

References

Mai J, et al. (2000) Cell surface complex of cathepsin B/annexin II tetramer in malignant progression. Biochim Biophys Acta. 1477(1-2): 215-30.Podgorski I, et al. (2003) Cathepsin B and its role(s) in cancer progression. Biochem Soc Symp. (70): 263-76.Yan S, et al. (2003) Molecular regulation of human cathepsin B: implication in pathologies. Biol Chem. 384(6): 845-54.Roshy S, et al. (2003) Pericellular cathepsin B and malignant progression. Cancer Metastasis Rev. 22(2-3): 271-86.

NCBI and Uniprot Product Information

• NCBI GI #: 4503139
• NCBI GeneID: 1508
• NCBI Accession #: NP_001899.1
• NCBI GenBank Nucleotide #: NM_001908.3
• UniProt Accession #: P07858; Q503A6; Q96D87; B3KQR5; B3KRR5
• Molecular Weight: 160,000
• NCBI Official Full Name: cathepsin B preproprotein
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: CTSB
• NCBI Official Synonym Symbols: APPS; CPSB
• NCBI Protein Information: cathepsin B; cathepsin B1; APP secretase; cysteine protease; amyloid precursor protein secretase
• UniProt Protein Name: Cathepsin B
• UniProt Gene Name: CTSB
• UniProt Synonym Gene Names: CPSB; APPS
• UniProt Entry Name: CATB_HUMAN