Rabbit anti-Mouse Cathepsin S Polyclonal Antibody | anti-CTSS antibody

Anti-Cathepsin S Antibody, Rabbit Polyclonal

Cat. Num. AAA259469

• Gene Names: Ctss; Cats
• Reactivity: Mouse
• Applications: Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Cathepsin S, Antibody; Anti-Cathepsin S Antibody, Rabbit Polyclonal; Cathepsin S Antibody, Rabbit PAb, Antigen Affinity Purified; cathepsin S; Anti-Cats Antibody; Anti-Ctss Antibody; anti-CTSS antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Cathepsin S
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CTSS antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Mouse Cathepsin S Protein ( AAB94925.1; Met1-Ile340). Cathepsin S specific IgG was purified by Mouse Cathepsin S affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

IP (Immunoprecipitation)

(mCTSS was immunoprecipitated using: Lane A:0.5 mg RAW 264.7 Whole Cell Lysate 4 uL anti-mCTSS rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-mCTSS rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size :37 kDa)

IHC (Immunohistochemisry)

(Immunochemical staining of Mouse Cathepsin S in Mouse brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of Mouse Cathepsin S in Mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-Cathepsin S rabbit polyclonal antibody at 1:500 dilutionLane A: RAW264.7 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:37 kDa)

Related Product Information for anti-CTSS antibody

Cathepsin S (CTSS), one of the lysosomal proteinases, has many important physiological functions in the nervous system, especially in process of extracellular matrix degradation and endocellular antigen presentation. CTSS is synthesized as inactive precursor of 331 amino acids consisting of a 15-aa signal peptide, a propeptide of 99 aa, and a mature polypeptide of 217 aa. It is activated in the lysosomes by a proteolytic cleavage of the propeptide. Cathepsin S is expressed in the lysosome of antigen presenting cells, primarily dendritic cells, B-cells and macrophages. Compared with other lysosomal cysteine proteases, cathepsin S has displayed some unique characteristics. Cathepsin S is most well known for its critical function in the proteolytic digestion of the invariant chain chaperone molecules, thus controlling antigen presentation to CD4+ T-cells by major histocompatibility complex (MHC) class II molecules or to NK1.1+ T-cells via CD1 molecules. Cathepsin S also appears to participate in direct processing of exogenous antigens for presentation by MHC class II to CD4+ T-cells, or in cross-presentation by MHC class I molecules to CD8+ T-cells. In addition, although direct evidence is still lacking, in its secreted form cathepsin S is implicated in degradation of the extracellular matrix, which may contribute to the pathology of a number of diseases, including arthritis, atherosclerosis, neurological diseases and chronic obstructive pulmonary disease.

References

Li. W. e. al. (2004. Cystein. proteas. cathepsi. . a. . ke. ste. i. antige. presentation. Dru. New. Perspect. 17(6). 357-63.Thurmon. RL. e. al. (2005. Cathepsi. . inhibitor. a. nove. immunomodulators. Cur. Opi. Investi. Drugs. 6(5). 473-82.Wan. DM. e. al. (2008. Cathepsi. . i. pathogenesi. o. neurologica. diseases. Zhejian. D. Xu. Xu. Ba. Y. Xu. Ban. 37(4). 422-6.

NCBI and Uniprot Product Information

• NCBI GI #: 2746723
• NCBI GeneID: 13040
• Molecular Weight: 38,475 Da
• NCBI Official Full Name: cathepsin S
• NCBI Official Synonym Full Names: cathepsin S
• NCBI Official Symbol: Ctss
• NCBI Official Synonym Symbols: Cats
• NCBI Protein Information: cathepsin S