FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U-87 cells using anti-CaV1.3 antibody (AAA46315).Overlay histogram showing U-87 cells stained with AAA46315 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaV1.3 Antibody (AAA46315,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CaV1.3 Polyclonal Antibody | anti-CaV1.3 antibody
Anti-CaV1.3 Antibody
Gene Names
CACNA1D; CACH3; CACN4; PASNA; SANDD; Cav1.3; CCHL1A2; CACNL1A2
Reactivity
Mouse, RatPredicted to work with: Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
CaV1.3, Antibody; Anti-CaV1.3 Antibody; Voltage-dependent L-type calcium channel subunit alpha-1D; alpha-1 polypeptide; CAC1D_HUMAN; CACH3; CACN4; CACNA 1D; CACNA1D; CACNL1A2; Calcium channel; Calcium channel L type alpha 1 polypeptide isoform 2; Calcium channel neuroendocrine/brain type alpha 1 subunit; Calcium channel voltage dependent L type alpha 1; Calcium channel voltage dependent L type alpha 1D subunit; Cav1.3; CCHL1A2; L type; PASNA; SANDD; Voltage dependent L type calcium channel subunit alpha 1D; Voltage gated calcium channel alpha 1 subunit; Voltage gated calcium channel alpha subunit Cav1.3; Voltage gated calcium channel subunit alpha Cav1.3; Voltage-gated calcium channel subunit alpha Cav1.3; calcium channel, voltage-dependent, L type, alpha 1D subunit; anti-CaV1.3 antibody
Reactivity
Mouse, Rat
Predicted to work with: Human
Predicted to work with: Human
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
2181
Applicable Applications for anti-CaV1.3 antibody
WB (Western Blot)
Immunogen
E Coli-derived human CaV1.3 recombinant protein (Position: M1-I180). Human CaV1.3 shares 99% and 98%amino acid (aa) sequence identity with mouse and rat CaV1.3, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U-87 cells using anti-CaV1.3 antibody (AAA46315).Overlay histogram showing U-87 cells stained with AAA46315 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaV1.3 Antibody (AAA46315,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U-87 cells using anti-CaV1.3 antibody (AAA46315).Overlay histogram showing U-87 cells stained with AAA46315 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaV1.3 Antibody (AAA46315,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of CAV1.3 using anti-CAV1.3 antibody (AAA46315).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Brain Tissue Lysate,Lane 2: Mouse Brain Tissue Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV1.3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CAV1.3 at approximately 245KD. The expected band size for CAV1.3 is at 245KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of CAV1.3 using anti-CAV1.3 antibody (AAA46315).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Brain Tissue Lysate,Lane 2: Mouse Brain Tissue Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV1.3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CAV1.3 at approximately 245KD. The expected band size for CAV1.3 is at 245KD.)
Related Product Information for anti-CaV1.3 antibody
Description: Rabbit IgG polyclonal antibody for Voltage-dependent L-type calcium channel subunit alpha-1D(CACNA1D) detection. Tested with WB in Human;Mouse;Rat.
Background: CACNA1D is also known as PASNA, SANDD or Cav1.3. Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, namely alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1D subunit. Several transcript variants encoding different isoforms have been found for this gene.
Background: CACNA1D is also known as PASNA, SANDD or Cav1.3. Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, namely alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1D subunit. Several transcript variants encoding different isoforms have been found for this gene.
References
1. Lieb A, et al. C-terminal modulatory domain controls coupling of voltage-sensing to pore opening in Cav1.3 L-type Ca(2+) channels. Biophys J, 2014 Apr 1. 2. Scholl UI, et al. Somatic and germline CACNA1D calcium channel mutations in aldosterone-producing adenomas and primary aldosteronism. Nat Genet, 2013 Sep.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
242,417 Da
NCBI Official Full Name
voltage-dependent L-type calcium channel subunit alpha-1D isoform a
NCBI Official Synonym Full Names
calcium voltage-gated channel subunit alpha1 D
NCBI Official Symbol
CACNA1D
NCBI Official Synonym Symbols
CACH3; CACN4; PASNA; SANDD; Cav1.3; CCHL1A2; CACNL1A2
NCBI Protein Information
voltage-dependent L-type calcium channel subunit alpha-1D
UniProt Protein Name
Voltage-dependent L-type calcium channel subunit alpha-1D
UniProt Gene Name
CACNA1D
UniProt Synonym Gene Names
CACH3; CACN4; CACNL1A2; CCHL1A2
UniProt Entry Name
CAC1D_HUMAN
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Product Notes
The CaV1.3 cacna1d (Catalog #AAA46315) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CaV1.3 Antibody reacts with Mouse, Rat Predicted to work with: Human and may cross-react with other species as described in the data sheet. AAA Biotech's CaV1.3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the CaV1.3 cacna1d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CaV1.3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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