IHC (Immunohistochemisry)
(Anti- CBS antibody, IHC(P)IHC(P): Human Liver Cancer Tissue)
anti-Human, Rat CBS Polyclonal Antibody | anti-CBS antibody
Anti-CBS Antibody
Gene Names
CBS; HIP4
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
CBS, Antibody; Anti-CBS Antibody; Cystathionine beta-synthase; AI047524; AI303044; Beta thionase; Beta-thionase; Cbs; Cbs cystathionine beta-synthase; CBS_HUMAN; Cystathionine beta synthase; EC 4.2.1.22; HIP 4; HIP4; Methylcysteine synthase; MGC18856; MGC18895; MGC37300; OTTHUMP00000109416; OTTHUMP00000109418; Serine sulfhydrase; cystathionine-beta-synthase; anti-CBS antibody
Reactivity
Human, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
551
Applicable Applications for anti-CBS antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human CBS recombinant protein (Position: A331-K551). Human CBS shares 83% amino acid (aa) sequence identity with both mouse and rat CBS.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(Anti- CBS antibody, IHC(P)IHC(P): Human Liver Cancer Tissue)
IHC (Immunohistochemisry)
(Anti- CBS antibody, IHC(P)IHC(P): Human Liver Cancer Tissue)
WB (Western Blot)
(Anti- CBS antibody, Western blottingAll lanes: Anti CBS at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Hela Whole Cell Lysate at 40ugLane 4: PANC Whole Cell Lysate at 40ugLane 5: Hepg2 Whole Cell Lysate at 40ugPredicted bind size: 60KDObserved bind size: 60KD)
WB (Western Blot)
(Anti- CBS antibody, Western blottingAll lanes: Anti CBS at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Hela Whole Cell Lysate at 40ugLane 4: PANC Whole Cell Lysate at 40ugLane 5: Hepg2 Whole Cell Lysate at 40ugPredicted bind size: 60KDObserved bind size: 60KD)
WB (Western Blot)
(Anti- CBS antibody, Western blottingAll lanes: Anti CBS at 0.5ug/mlWB: Recombinant human CBS Protein 0.5ngPredicted bind size: 42KDObserved bind size: 42KD)
WB (Western Blot)
(Anti- CBS antibody, Western blottingAll lanes: Anti CBS at 0.5ug/mlWB: Recombinant human CBS Protein 0.5ngPredicted bind size: 42KDObserved bind size: 42KD)
Related Product Information for anti-CBS antibody
Description: Rabbit IgG polyclonal antibody for Cystathionine beta-synthase(CBS) detection. Tested with WB, IHC-P in Human;Rat.
Background: Cystathionine-beta-synthase, also known as CBS, is an enzyme that in humans is encoded by the CBS gene. It is mapped to 21q22.3 and contains 23 exons, ranging in size from 42 to 299 bp. CBS catalyzes the first step of the transsulfuration pathway, from homocysteine to cystathionine. It uses the cofactor pyridoxal-phosphate (PLP) and can be allosterically regulated by effectors such as the ubiquitous cofactor S-adenosyl-L-methionine (adoMet). This enzyme belongs to the family of lyases, to be specific, the hydro-lyases, which cleave carbon-oxygen bonds. CBS is a multidomain enzyme composed of an N-terminal enzymatic domain and two CBS domains. The CBS gene is the most common locus for mutations associated with homocystinuria.
Background: Cystathionine-beta-synthase, also known as CBS, is an enzyme that in humans is encoded by the CBS gene. It is mapped to 21q22.3 and contains 23 exons, ranging in size from 42 to 299 bp. CBS catalyzes the first step of the transsulfuration pathway, from homocysteine to cystathionine. It uses the cofactor pyridoxal-phosphate (PLP) and can be allosterically regulated by effectors such as the ubiquitous cofactor S-adenosyl-L-methionine (adoMet). This enzyme belongs to the family of lyases, to be specific, the hydro-lyases, which cleave carbon-oxygen bonds. CBS is a multidomain enzyme composed of an N-terminal enzymatic domain and two CBS domains. The CBS gene is the most common locus for mutations associated with homocystinuria.
References
1. Munke, M., Kraus, J., Watkins, P., Tanzi, R., Gusella, J., Millington Ward, A., Watson, M., Francke, U.Homocys 2. tinuria gene on human chromosome 21 mapped with cloned cystathionine beta-synthase probe and in situ hybridization of other chromosome 21 probes. (Abstract)Cytogenet. Cell Genet. 40: 706-707, 1985. 3. Kraus, J. P., Oliveriusova, J., Sokolova, J., Kraus, E., Vlcek, C., de Franchis, R., Maclean, K. N., Bao, L., Bukovska, G., Patterson, D., Paces, V., Ansorge, W., Kozich, V.The human cystathionine beta-synthase (CBS) gene: complete sequence, alternative splicing, and polymorphisms.Genomics 52: 312-324, 1998. 4. Kruger, W. D., Cox, D. R.A yeast system for expression of human cystathionine beta-synthase: structural and functional conservation of the human and yeast genes.Proc. Nat. Acad. Sci. 91: 6614-6618, 1994.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,863 Da
NCBI Official Full Name
cystathionine beta-synthase isoform 1
NCBI Official Synonym Full Names
cystathionine-beta-synthase
NCBI Official Symbol
CBS
NCBI Official Synonym Symbols
HIP4
NCBI Protein Information
cystathionine beta-synthase
UniProt Protein Name
Cystathionine beta-synthase
UniProt Gene Name
CBS
UniProt Entry Name
CBS_HUMAN
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Product Notes
The CBS cbs (Catalog #AAA46431) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CBS Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CBS can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CBS cbs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CBS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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