IP (Immunoprecipitation)
(CCDC90B was immunoprecipitated using:Lane A:0.5 mg U-251MG Whole Cell Lysate4 uL anti-CCDC90B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CCDC90B rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :26 kDa)
Rabbit anti-Human CCDC90B Polyclonal Antibody | anti-CCDC90B antibody
Anti-CCDC90B Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CCDC90B, Antibody; Anti-CCDC90B Antibody, Rabbit Polyclonal; CCDC90B Antibody, Rabbit PAb, Antigen Affinity Purified; coiled-coil domain containing 90B; Anti-MDS011 Antibody; Anti-MDS025 Antibody; anti-CCDC90B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CCDC90B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-CCDC90B antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
E Coli-derived Human CCDC90B fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human CCDC90B fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(CCDC90B was immunoprecipitated using:Lane A:0.5 mg U-251MG Whole Cell Lysate4 uL anti-CCDC90B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CCDC90B rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :26 kDa)
IP (Immunoprecipitation)
(CCDC90B was immunoprecipitated using:Lane A:0.5 mg U-251MG Whole Cell Lysate4 uL anti-CCDC90B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CCDC90B rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :26 kDa)
WB (Western Blot)
(Anti-CCDC90B rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: U-251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-CCDC90B rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: U-251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-CCDC90B antibody
The CCDC90B (coiled-coil domain containing 90B) gene, located on 11q14.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. An important paralog of this gene is MCUR1. The encoded protein is characterized by the presence of a domain of the unknown function, DUF1640. This domain is a characteristic of the entire protein except for the first twenty-three amino acid residues-MNSRQAWRLFLSQGRGDRWVSRP-which are a mitochondrial targeting site and cleaved. The protein is predicted to contain at least three specific phosphorylation sites: Protein Kinase C Phosphorylation sites, Casein Kinase II Phosphorylation sites, and cAMP/cGMP Dependent Phosphorylation sites. CCDC90B is commonly expressed in the testis, adrenal glands, and 25 other tissues. Diseases associated with CCDC90B include Oculoauricular Syndrome and Osteochondrosis.
References
Xiao T, et al. (2018) Estrogen-regulated feedback loop limits the efficacy of estrogen receptor-targeted breast cancer therapy. Proc Natl Acad Sci U S A 115 (31): 7869-7878.Tomar D, et al. (2016) Mcur1 is a scaffold factor for the mcu complex function and promotes mitochondrial bioenergetics. Cell Rep 15 (8): 1673-1685.Adlakha J, et al. (2019) Characterization of mcu-binding proteins mcur1 and ccdc90b-representatives of a protein family conserved in prokaryotes and eukaryotic organelles. Structure 27 (3): 464-475.e466.
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Product Notes
The CCDC90B (Catalog #AAA256128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CCDC90B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCDC90B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CCDC90B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCDC90B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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