Rabbit anti-Rat Ccnd3 Polyclonal Antibody | anti-Ccnd3 antibody

(RAT) Ccnd3 Antibody (N-term)

Cat. Num. AAA288837

• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: Ccnd3, Antibody; (RAT) Ccnd3 Antibody (N-term); G1/S-specific cyclin-D3; Ccnd3; anti-Ccnd3 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This rat Ccnd3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 33-66 amino acids from the N-terminal region of rat Ccnd3.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 293

• Applicable Applications for anti-Ccnd3 antibody: ELISA, WB (Western Blot)
• Function: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Shows transcriptional coactivator activity with ATF5 independently of CDK4.
• Cellular Location: Nucleus {ECO:0000250|UniProtKB:P30281}. Cytoplasm {ECO:0000250|UniProtKB:P30281}
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of lysate from rat spleen tissue, using Ccnd3 Antibody (N-term). AAA288837 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

WB (Western Blot)

(Western blot analysis of lysates from Hela cell line, mouse spleen, rat lung, rat stomach tissue (from left to right), using Ccnd3 Antibody (N-term). AAA288837 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Related Product Information for anti-Ccnd3 antibody

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Product Categories/Family for anti-Ccnd3 antibody

Cancer; Cell biology

References

Hosokawa Y.,et al.Gene 147:249-252(1994).
Yang M.,et al.Gene 181:153-159(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 6978619
• NCBI GeneID: 25193
• NCBI Accession #: NP_036898.1
• NCBI GenBank Nucleotide #: NM_012766.1
• UniProt Accession #: P48961; Q63628
• Molecular Weight: 32434
• NCBI Official Full Name: G1/S-specific cyclin-D3
• NCBI Official Synonym Full Names: cyclin D3
• NCBI Official Symbol: Ccnd3
• NCBI Protein Information: G1/S-specific cyclin-D3
• UniProt Protein Name: G1/S-specific cyclin-D3
• UniProt Gene Name: Ccnd3
• UniProt Entry Name: CCND3_RAT