FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using CCT3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit CCT3 Polyclonal Antibody | anti-CCT3 antibody
CCT3 Antibody (C-term)
Gene Names
CCT3; CCTG; PIG48; TRIC5; CCT-gamma; TCP-1-gamma
Reactivity
Human (Predicted Reactivity: Bovine, Monkey)
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CCT3, Antibody; CCT3 Antibody (C-term); T-complex protein 1 subunit gamma; TCP-1-gamma; CCT-gamma; hTRiC5; CCT3; CCTG; TRIC5; anti-CCT3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CCT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 509-536 amino acids from the C-terminal region of human CCT3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
509-536
Sequence Length
507
Applicable Applications for anti-CCT3 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using CCT3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using CCT3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of CCT3 Antibody (C-term) in K562 cell line lysates (35ug/lane). CCT3 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CCT3 Antibody (C-term) in K562 cell line lysates (35ug/lane). CCT3 (arrow) was detected using the purified Pab.)
Related Product Information for anti-CCT3 antibody
CCT3 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.
Product Categories/Family for anti-CCT3 antibody
References
Zebol,J.R., Int. J. Biochem. Cell Biol. 41 (4), 822-827 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60534
NCBI Official Full Name
T-complex protein 1 subunit gamma isoform c
NCBI Official Synonym Full Names
chaperonin containing TCP1, subunit 3 (gamma)
NCBI Official Symbol
CCT3
NCBI Official Synonym Symbols
CCTG; PIG48; TRIC5; CCT-gamma; TCP-1-gamma
NCBI Protein Information
T-complex protein 1 subunit gamma
UniProt Protein Name
T-complex protein 1 subunit gamma
UniProt Gene Name
CCT3
UniProt Synonym Gene Names
CCTG; TRIC5; TCP-1-gamma
UniProt Entry Name
TCPG_HUMAN
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Product Notes
The CCT3 cct3 (Catalog #AAA289548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 509-536. The CCT3 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's CCT3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CCT3 cct3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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