WB (Western Blot)
(Detection of human and mouse CCT4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCT4 antibody AAA212889 (lot AAA212889-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse CCT4 Polyclonal Antibody | anti-CCT4 antibody
Rabbit anti-CCT4 Antibody, Affinity Purified
Gene Names
CCT4; SRB; Cctd; CCT-DELTA; MGC126164; MGC126165
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CCT4, Antibody; Rabbit anti-CCT4 Antibody, Affinity Purified; chaperonin containing TCP1, subunit 4 (delta); TCP-1-delta; T-complex protein 1 subunit delta; SRB; chaperonin containing t-complex polypeptide 1, delta subunit; CCT-DELTA; Cctd; stimulator of TAR RNA-binding; chaperonin containing TCP1 subunit 4; anti-CCT4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CCT4 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 489 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse CCT4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCT4 antibody AAA212889 (lot AAA212889-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse CCT4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCT4 antibody AAA212889 (lot AAA212889-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CCT4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCT4 antibody AAA212889 (lot AAA212889-1) used for IP at 6 ug per reaction. CCT4 was also immunoprecipitated by rabbit anti-CCT4 antibody For blotting immunoprecipitated CCT4, AAA212889 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CCT4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCT4 antibody AAA212889 (lot AAA212889-1) used for IP at 6 ug per reaction. CCT4 was also immunoprecipitated by rabbit anti-CCT4 antibody For blotting immunoprecipitated CCT4, AAA212889 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-CCT4 antibody
Chaperonin containing TCP1 subunit 4 (delta) or CCT4 is a molecular chaperone. It assists with the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, it may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. It is also known to play a role, in vitro, in the folding of actin and tubulin [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P50991].
Product Categories/Family for anti-CCT4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,924 Da
NCBI Official Full Name
T-complex protein 1 subunit delta
NCBI Official Synonym Full Names
chaperonin containing TCP1, subunit 4 (delta)
NCBI Official Symbol
CCT4
NCBI Official Synonym Symbols
SRB; Cctd; CCT-DELTA; MGC126164; MGC126165
NCBI Protein Information
T-complex protein 1 subunit delta; TCP-1-delta; OTTHUMP00000201357; stimulator of TAR RNA-binding; chaperonin containing t-complex polypeptide 1, delta subunit
UniProt Protein Name
T-complex protein 1 subunit delta
UniProt Gene Name
CCT4
UniProt Synonym Gene Names
CCTD; SRB; TCP-1-delta
UniProt Entry Name
TCPD_HUMAN
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Product Notes
The CCT4 cct4 (Catalog #AAA212889) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CCT4 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CCT4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CCT4 cct4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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