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product-image-AAA200431_WB10.jpg WB (Western Blot) (WB Suggested Anti-CCT5 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human brain)

Rabbit CCT5 Polyclonal Antibody | anti-CCT5 antibody

CCT5 antibody - N-terminal region

Gene Names
CCT5; CCTE; HEL-S-69; PNAS-102; CCT-epsilon; TCP-1-epsilon
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
CCT5, Antibody; CCT5 antibody - N-terminal region; anti-CCT5 antibody
Ordering
Host
Rabbit
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: NDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAE
Sequence Length
541
Applicable Applications for anti-CCT5 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human CCT5
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(WB Suggested Anti-CCT5 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human brain)

product-image-AAA200431_WB10.jpg WB (Western Blot) (WB Suggested Anti-CCT5 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human brain)

WB (Western Blot)

(Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceApplication: Western blottingSpecies+tissue/cell type: Mouse WT brain and Rat brainHow many ug's of tissue/cell lysate run on the gel: 1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary antibody dilution: 2ug/mlSecondary antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary antibody dilution: 1: 20,000)

product-image-AAA200431_WB11.jpg WB (Western Blot) (Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceApplication: Western blottingSpecies+tissue/cell type: Mouse WT brain and Rat brainHow many ug's of tissue/cell lysate run on the gel: 1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary antibody dilution: 2ug/mlSecondary antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary antibody dilution: 1: 20,000)

WB (Western Blot)

(Lanes:1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution:2ug/mlSecondary Antibody:IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution:1: 20,000Gene Name:CCT5Submitted by:Dr. Yuzhi Chen, University of Arkansas for Medical ScienceCCT5 is supported by BioGPS gene expression data to be expressed in HEK293)

product-image-AAA200431_WB13.jpg WB (Western Blot) (Lanes:1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution:2ug/mlSecondary Antibody:IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution:1: 20,000Gene Name:CCT5Submitted by:Dr. Yuzhi Chen, University of Arkansas for Medical ScienceCCT5 is supported by BioGPS gene expression data to be expressed in HEK293)

IHC (Immunohistochemistry)

(Rabbit Anti-CCT5 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human heart TissueObserved Staining: CytoplasmicPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

product-image-AAA200431_IHC15.jpg IHC (Immunohistochemistry) (Rabbit Anti-CCT5 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human heart TissueObserved Staining: CytoplasmicPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
Related Product Information for anti-CCT5 antibody
This is a rabbit polyclonal antibody against CCT5. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: CCT5 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Product Categories/Family for anti-CCT5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60kDa
NCBI Official Full Name
T-complex protein 1 subunit epsilon isoform a
NCBI Official Synonym Full Names
chaperonin containing TCP1 subunit 5
NCBI Official Symbol
CCT5
NCBI Official Synonym Symbols
CCTE; HEL-S-69; PNAS-102; CCT-epsilon; TCP-1-epsilon
NCBI Protein Information
T-complex protein 1 subunit epsilon
UniProt Protein Name
T-complex protein 1 subunit epsilon
UniProt Gene Name
CCT5
UniProt Synonym Gene Names
CCTE; KIAA0098; TCP-1-epsilon
UniProt Entry Name
TCPE_HUMAN

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Product Notes

The CCT5 cct5 (Catalog #AAA200431) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCT5 antibody - N-terminal region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's CCT5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CCT5 cct5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: NDGATILSMM DVDHQIAKLM VELSKSQDDE IGDGTTGVVV LAGALLEEAE. It is sometimes possible for the material contained within the vial of "CCT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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