IF (Immunofluorescence)
(AAA325202 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human CD1E Polyclonal Antibody | anti-CD1E antibody
CD1E Antibody
Gene Names
CD1E; R2; CD1A
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CD1E, Antibody; CD1E Antibody; CD_antigen=CD1e; CD1A; CD1e; CD1e antigen; CD1E antigen, e polypeptide; CD1e molecule; CD1E_HUMAN; Differentiation antigen CD1-alpha-3; hCD1e; Leukocyte differentiation antigen; R2; R2G1; sCD1e; soluble; T-cell surface glycoprotein CD1e; T-cell surface glycoprotein CD1e, membrane-associated; T-cell surface glycoprotein CD1e, soluble; anti-CD1E antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CD1E antibody detects endogenous levels of total CD1E
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
376
Applicable Applications for anti-CD1E antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Endosome; Golgi Apparatus; Lysosome
Tissue Specificity
Expressed on cortical thymocytes, dendritic cells, Langerhans cells, on certain T-cell leukemias, and in various other tissues.
Predicted Cross Reactivity
Pig, Dog
Similarity
Pig (80%), Dog (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA325202 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA325202 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HuvEc cells using CD1E antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HuvEc cells using CD1E antibody.)
Related Product Information for anti-CD1E antibody
Function: T-cell surface glycoprotein CD1e, soluble binds diacetylated lipids, including phosphatidyl inositides and diacylated sulfoglycolipids, and is required for the presentation of glycolipid antigens on the cell surface. The membrane-associated form is not active.
Subunit Structure: Heterodimer with B2M (beta-2-microglobulin). The association with B2M appears to be facilitated by the presence of the propeptide.
Post-translational Modifications: Mono-ubiquitinated. Proteolytically cleaved in late endosomes to yield a soluble form.
Subunit Structure: Heterodimer with B2M (beta-2-microglobulin). The association with B2M appears to be facilitated by the presence of the propeptide.
Post-translational Modifications: Mono-ubiquitinated. Proteolytically cleaved in late endosomes to yield a soluble form.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 36 kD
Predicted: 44 kDa
Predicted: 44 kDa
NCBI Official Full Name
T-cell surface glycoprotein CD1e, membrane-associated isoform b
NCBI Official Synonym Full Names
CD1e molecule
NCBI Official Symbol
CD1E
NCBI Official Synonym Symbols
R2; CD1A
NCBI Protein Information
T-cell surface glycoprotein CD1e, membrane-associated
UniProt Protein Name
T-cell surface glycoprotein CD1e, membrane-associated
UniProt Gene Name
CD1E
UniProt Synonym Gene Names
hCD1e; sCD1e
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Product Notes
The CD1E cd1e (Catalog #AAA325202) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD1E Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD1E can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CD1E cd1e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD1E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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