Rabbit anti-Human, Mouse CD253/TRAIL Polyclonal Antibody | anti-CD253/TRAIL antibody

Rabbit anti CD253/TRAIL/ (TNFSF10) (IN1) Polyclonal Antibody

Cat. Num. AAA71329

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified by Epitope Affinity Purification
• Synonyms: CD253/TRAIL, Antibody; Rabbit anti CD253/TRAIL/ (TNFSF10) (IN1) Polyclonal Antibody; TNFSF10; APO2L; anti-CD253/TRAIL antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ~~34 kDa of human TRAIL protein.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.

• Applicable Applications for anti-CD253/TRAIL antibody: WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA
• Preparation: A synthetic peptide corresponding to the N-terminus of TRAIL protein (from 115aa-140aa). This sequence is identical to human and mouse.
• Positive Control: Raji cell
• Cellular location: Membrane/cytoplasmic
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

WB (Western Blot)

(WB: The cell lysate derived from Raji was separated in 10% SDSPAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti CD253/TRAIL (IN1) (Cat#AAA71329) at 1:500. An immunoreactive band is observed at ~34 kDa.)

Related Product Information for anti-CD253/TRAIL antibody

The TRAIL (TNF related apoptosis induced ligand) or Apo 2L /CD253 protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. The ligand binding to its receptors DR4/DR5 initiates apoptosis of tumor cells. TRAIL is expressed predominantly in spleen, lung, and prostate but also in many other tissues. TRAIL is a type II membrane protein from the TNF family. The human form of the protein is 281 amino acids in length, whereas the murine form is 291 amino acids. The two forms share 65% identity. TRAIL protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.

References

Pitti, R.M., Marsters, S.A., Ruppert, S., Donahue, C.J., Moore, A. and Ashkenazi, A. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem. 271 (22), 12687-12690 (1996)