FCM/FACS (Flow Cytometry)
(CD27 Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CD27 Polyclonal Antibody | anti-CD27 antibody
CD27 Antibody (C-term)
Gene Names
CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CD27, Antibody; CD27 Antibody (C-term); CD27 antigen; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; CD27; TNFRSF7; anti-CD27 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CD27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from the C-terminal region of human CD27.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.49 (varies by lot)
Sequence Positions
196-225
Sequence Length
260
Applicable Applications for anti-CD27 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CD27 Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CD27 Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of CD27 (arrow) using rabbit polyclonal CD27 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD27 gene.)
WB (Western Blot)
(Western blot analysis of CD27 (arrow) using rabbit polyclonal CD27 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD27 gene.)
WB (Western Blot)
(CD27 Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the CD27 antibody detected the CD27 protein (arrow).)
WB (Western Blot)
(CD27 Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the CD27 antibody detected the CD27 protein (arrow).)
Related Product Information for anti-CD27 antibody
The protein encoded by this gene is a member of the
TNF-receptor superfamily. This receptor is required for generation
and long-term maintenance of T cell immunity. It binds to ligand
CD70, and plays a key role in regulating B-cell activation and
immunoglobulin synthesis. This receptor transduces signals that
lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins
TRAF2 and TRAF5 have been shown to mediate the signaling process of
this receptor. CD27-binding protein (SIVA), a proapoptotic protein,
can bind to this receptor and is thought to play an important role
in the apoptosis induced by this receptor.
TNF-receptor superfamily. This receptor is required for generation
and long-term maintenance of T cell immunity. It binds to ligand
CD70, and plays a key role in regulating B-cell activation and
immunoglobulin synthesis. This receptor transduces signals that
lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins
TRAF2 and TRAF5 have been shown to mediate the signaling process of
this receptor. CD27-binding protein (SIVA), a proapoptotic protein,
can bind to this receptor and is thought to play an important role
in the apoptosis induced by this receptor.
Product Categories/Family for anti-CD27 antibody
References
Jiang, J., et al. J. Clin. Immunol. 30(4):566-573(2010)
Arimoto-Miyamoto, K., et al. Immunology 130(1):137-149(2010)
Mizuochi, T., et al. J. Interferon Cytokine Res. 30(4):243-252(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Neron, S., et al. Arch. Immunol. Ther. Exp. (Warsz.) 57(6):447-458(2009)
Arimoto-Miyamoto, K., et al. Immunology 130(1):137-149(2010)
Mizuochi, T., et al. J. Interferon Cytokine Res. 30(4):243-252(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Neron, S., et al. Arch. Immunol. Ther. Exp. (Warsz.) 57(6):447-458(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29137
NCBI Official Full Name
CD27 antigen
NCBI Official Synonym Full Names
CD27 molecule
NCBI Official Symbol
CD27
NCBI Official Synonym Symbols
T14; S152; Tp55; TNFRSF7; S152. LPFS2
NCBI Protein Information
CD27 antigen
UniProt Protein Name
CD27 antigen
UniProt Gene Name
CD27
UniProt Synonym Gene Names
TNFRSF7
UniProt Entry Name
CD27_HUMAN
Similar Products
Product Notes
The CD27 cd27 (Catalog #AAA285755) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 196-225. The CD27 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD27 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD27 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.