WB (Western Blot)
(Detection of human CD3D by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD3D antibody AAA213130 (lot AAA213130-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human CD3D Polyclonal Antibody | anti-CD3D antibody
Rabbit anti-CD3D Antibody, Affinity Purified
Gene Names
CD3D; T3D; IMD19; CD3-DELTA
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CD3D, Antibody; Rabbit anti-CD3D Antibody, Affinity Purified; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 delta chain; T3D; OKT3, delta chain; IMD19; CD3d molecule, delta (CD3-TCR complex); CD3d antigen, delta polypeptide (TiT3 complex); CD3 delta; CD antigen CD3d; CD3 antigen, delta subunit; CD3-DELTA; CD3d molecule; anti-CD3D antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CD3D antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 121 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD3D by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD3D antibody AAA213130 (lot AAA213130-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human CD3D by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD3D antibody AAA213130 (lot AAA213130-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CD3D by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CD3D antibody AAA213130 (lot AAA213130-1) used for IP at 6 ug per reaction. CD3D was also immunoprecipitated by rabbit anti-CD3D antibody BL20487. For blotting immunoprecipitated CD3D, AAA213130 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CD3D by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CD3D antibody AAA213130 (lot AAA213130-1) used for IP at 6 ug per reaction. CD3D was also immunoprecipitated by rabbit anti-CD3D antibody BL20487. For blotting immunoprecipitated CD3D, AAA213130 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-CD3D antibody
The protein encoded for by CD3D, T-cell surface glycoprotein CD3 delta chain, is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. CD3 delta chain, along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells [taken from NCBI Entrez Gene (Gene ID: 915)].
Product Categories/Family for anti-CD3D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,484 Da
NCBI Official Full Name
T-cell surface glycoprotein CD3 delta chain isoform A
NCBI Official Synonym Full Names
CD3d molecule
NCBI Official Symbol
CD3D
NCBI Official Synonym Symbols
T3D; IMD19; CD3-DELTA
NCBI Protein Information
T-cell surface glycoprotein CD3 delta chain
UniProt Protein Name
T-cell surface glycoprotein CD3 delta chain
UniProt Gene Name
CD3D
UniProt Synonym Gene Names
T3D
UniProt Entry Name
CD3D_HUMAN
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Product Notes
The CD3D cd3d (Catalog #AAA213130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD3D Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD3D can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CD3D cd3d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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