IP (Immunoprecipitation)
(CD46 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-CD46 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD46 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 62 kDa)
Rabbit anti-Human CD46 Polyclonal Antibody | anti-CD46 antibody
Anti-CD46 Antibody, Rabbit Polyclonal
Gene Names
CD46; MCP; TLX; AHUS2; MIC10; TRA2.10
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CD46, Antibody; Anti-CD46 Antibody, Rabbit Polyclonal; CD46 Antibody, Rabbit PAb, Antigen Affinity Purified; CD46 molecule, complement regulatory protein; Anti-AHUS2 Antibody; Anti-MCP Antibody; Anti-MIC10 Antibody; Anti-TLX Antibody; Anti-TRA2.10 Antibody; anti-CD46 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CD46
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD46 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human CD46 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human CD46 (rh CD46). CD46 specific IgG was purified by Human CD46 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(CD46 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-CD46 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD46 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 62 kDa)
IP (Immunoprecipitation)
(CD46 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-CD46 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD46 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 62 kDa)
WB (Western Blot)
(Anti-CD46 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:62 kDa)
WB (Western Blot)
(Anti-CD46 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:62 kDa)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD46 in human placenta with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of trophoblast.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD46 in human placenta with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of trophoblast.)
Related Product Information for anti-CD46 antibody
CD46, also known as Membrane Cofactor Protein (MCP), is a complement regulatory protein. CD46 is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 is expressed in white blood cells, platelets, epithelial cells, and fibroblasts. Human CD46 shares 5% amino acid sequence identity with mouse and rat CD46. The importance of CD46 to complement regulation is underscored by the observation that genetic loss of CD46 leads to development of atypical hemolytic-uremic syndrome (aHUS), a disease characterized by uncontrolled complement activation. CD46 is implicated in the development and/or progression of selected cancer types.
Product Categories/Family for anti-CD46 antibody
References
Lublin D.M., et al., (1988), Molecular cloning and chromosomal localization of human membrane cofactor protein (MCP). Evidence for inclusion in the multigene family of complement-regulatory proteins. J. Exp. Med. 168:181-194.Purcell D.F., et al., (1991), Alternatively spliced RNAs encode several isoforms of CD46 (MCP), a regulator of complement activation.Immunogenetics 33:335-344.Post T.W., et al., (1991), Membrane cofactor protein of the complement system: alternative splicing of serine/threonine/proline-rich exons and cytoplasmic tails produces multiple isoforms that correlate with protein phenotype.J. Exp. Med. 174:93-102.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
36,826 Da
NCBI Official Full Name
CD46 antigen, complement regulatory protein, isoform CRA_i
NCBI Official Synonym Full Names
CD46 molecule
NCBI Official Symbol
CD46
NCBI Official Synonym Symbols
MCP; TLX; AHUS2; MIC10; TRA2.10
NCBI Protein Information
membrane cofactor protein
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Product Notes
The CD46 (Catalog #AAA255548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD46 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD46 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD46 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD46, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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