WB (Western Blot)
(Detection of human CD48 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD48 antibody AAA212690 (lot AAA212690-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human CD48 Polyclonal Antibody | anti-CD48 antibody
Rabbit anti-CD48 Antibody, Affinity Purified
Gene Names
CD48; BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CD48, Antibody; Rabbit anti-CD48 Antibody, Affinity Purified; TCT.1; leukocyte antigen MEM-102; mCD48; MEM-102; signaling lymphocytic activation molecule 2; SLAMF2; CD48 antigen; SLAM family member 2; CD48 antigen (B-cell membrane protein); CD antigen CD48; B-lymphocyte activation marker BLAST-1; BLAST1; BLAST; BCM1 surface antigen; BCM1; hCD48; CD48 molecule; anti-CD48 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CD48 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 150 and 200
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD48 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD48 antibody AAA212690 (lot AAA212690-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human CD48 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD48 antibody AAA212690 (lot AAA212690-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CD48 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD48 antibody AAA212690 (lot AAA212690-1) used for IP at 6 ug per reaction. CD48 was inefficiently immunoprecipitated by rabbit anti-CD48 antibody BL16585. For blotting immunoprecipitated CD48, AAA212690 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CD48 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD48 antibody AAA212690 (lot AAA212690-1) used for IP at 6 ug per reaction. CD48 was inefficiently immunoprecipitated by rabbit anti-CD48 antibody BL16585. For blotting immunoprecipitated CD48, AAA212690 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-CD48 antibody
CD48 is a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. CD48 does not have a transmembrane domain, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor [taken from NCBI Entrez Gene (Gene ID: 962)].
Product Categories/Family for anti-CD48 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,429 Da
NCBI Official Full Name
CD48 antigen isoform 1
NCBI Official Synonym Full Names
CD48 molecule
NCBI Official Symbol
CD48
NCBI Official Synonym Symbols
BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
NCBI Protein Information
CD48 antigen
UniProt Protein Name
CD48 antigen
UniProt Gene Name
CD48
UniProt Synonym Gene Names
BCM1; BLAST1; SLAMF2
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Product Notes
The CD48 cd48 (Catalog #AAA212690) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD48 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD48 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CD48 cd48 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD48, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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