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FCM/FACS (Flow Cytometry)
(CD55 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CD55 Polyclonal Antibody | anti-CD55 antibody
CD55 Antibody (N-term)
Gene Names
CD55; CR; TC; DAF; CROM
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CD55, Antibody; CD55 Antibody (N-term); Complement decay-accelerating factor; CD55; CR; DAF; anti-CD55 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CD55 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human CD55.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
51-79
Sequence Length
381
Applicable Applications for anti-CD55 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CD55 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CD55 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(CD55 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CD55 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(CD55 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CD55 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD55 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD55 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(CD55 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CD55 antibody detected the CD55 protein (arrow).)
WB (Western Blot)
(CD55 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CD55 antibody detected the CD55 protein (arrow).)
Related Product Information for anti-CD55 antibody
This gene encodes a protein involved in the regulation of
the complement cascade. The encoded glycoprotein is also known as
the decay-accelerating factor (DAF); binding of DAF to complement
proteins accelerates their decay, disrupting the cascade and
preventing damage to host cells. Antigens present on the DAF
glycoprotein constitute the Cromer blood group system (CROM). Two
alternatively spliced transcripts encoding different proteins have
been identified. The predominant transcript encodes a
membrane-bound protein expressed on cells exposed to plasma
component proteins but an alternatively spliced transcript produces
a soluble protein present at much lower levels. Additional,
alternatively spliced transcript variants have been described, but
their biological validity has not been determined. [provided by
RefSeq].
the complement cascade. The encoded glycoprotein is also known as
the decay-accelerating factor (DAF); binding of DAF to complement
proteins accelerates their decay, disrupting the cascade and
preventing damage to host cells. Antigens present on the DAF
glycoprotein constitute the Cromer blood group system (CROM). Two
alternatively spliced transcripts encoding different proteins have
been identified. The predominant transcript encodes a
membrane-bound protein expressed on cells exposed to plasma
component proteins but an alternatively spliced transcript produces
a soluble protein present at much lower levels. Additional,
alternatively spliced transcript variants have been described, but
their biological validity has not been determined. [provided by
RefSeq].
Product Categories/Family for anti-CD55 antibody
References
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Gustafsson, D.J., et al. Virology 405(2):474-482(2010)
Alegretti, A.P., et al. Cell. Immunol. 265(2):127-132(2010)
Kim, Y., et al. Ann. Clin. Lab. Sci. 40(3):226-232(2010)
Storry, J.R., et al. Transfusion 43(3):340-344(2003)
Gustafsson, D.J., et al. Virology 405(2):474-482(2010)
Alegretti, A.P., et al. Cell. Immunol. 265(2):127-132(2010)
Kim, Y., et al. Ann. Clin. Lab. Sci. 40(3):226-232(2010)
Storry, J.R., et al. Transfusion 43(3):340-344(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41400
NCBI Official Full Name
complement decay-accelerating factor isoform 1 preproprotein
NCBI Official Synonym Full Names
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
NCBI Official Symbol
CD55
NCBI Official Synonym Symbols
CR; TC; DAF; CROM
NCBI Protein Information
complement decay-accelerating factor
UniProt Protein Name
Complement decay-accelerating factor
UniProt Gene Name
CD55
UniProt Synonym Gene Names
CR; DAF
UniProt Entry Name
DAF_HUMAN
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Product Notes
The CD55 cd55 (Catalog #AAA284097) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 51-79. The CD55 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD55 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD55 cd55 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD55, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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