IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug CD71/Transferrin Receptor antibody. Western blot was performed from the immunoprecipitate using CD71/Transferrin Receptor antibody at a dilution of 1:1000.)
Rabbit anti-Human, Mouse CD71/Transferrin Receptor Polyclonal Antibody | anti-CD71 antibody
CD71/Transferrin Receptor Rabbit pAb
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity purification
Synonyms
CD71/Transferrin Receptor, Antibody; CD71/Transferrin Receptor Rabbit pAb; TFRC; CD71; IMD46; T9; TFR; TFR1; TR; TRFR; p90; transferrin receptor; anti-CD71 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.01% thiomersal, 50% glycerol, pH7.3.
Applicable Applications for anti-CD71 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Positive Samples
HeLa, Rat spleen
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-250 of human CD71/Transferrin Receptor (NP_003225.2).
Cellular Location
Cell membrane, Melanosome, Secreted, Single-pass type II membrane protein
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug CD71/Transferrin Receptor antibody. Western blot was performed from the immunoprecipitate using CD71/Transferrin Receptor antibody at a dilution of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug CD71/Transferrin Receptor antibody. Western blot was performed from the immunoprecipitate using CD71/Transferrin Receptor antibody at a dilution of 1:1000.)
WB (Western Blot)
(Western blot analysis of Rat spleen, using CD71/TransferrinReceptor antibody at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.)
WB (Western Blot)
(Western blot analysis of Rat spleen, using CD71/TransferrinReceptor antibody at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.)
WB (Western Blot)
(Western blot analysis of HeLa, using CD71/TransferrinReceptor antibody at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of HeLa, using CD71/TransferrinReceptor antibody at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Related Product Information for anti-CD71 antibody
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.
Product Categories/Family for anti-CD71 antibody
NCBI and Uniprot Product Information
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Product Notes
The CD71 (Catalog #AAA282227) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD71/Transferrin Receptor Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD71/Transferrin Receptor can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CD71 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD71/Transferrin Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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