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Application Data
(Li, Lin, et al. "Telekin suppresses human hepatocellular carcinoma cells in vitro by inducing G 2/M phase arrest via the p38 MAPK signaling pathway." Acta Pharmacologica Sinica 35.10 (2014): 1311.)
Rabbit Cdc25A Polyclonal Antibody | anti-Cdc25A antibody
Cdc25A (Phospho-Ser75) Rabbit Polyclonal Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Cdc25A, Antibody; Cdc25A (Phospho-Ser75) Rabbit Polyclonal Antibody; CDC25A; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A; BH6842; anti-Cdc25A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Phospho-Cdc25A (S75) Polyclonal Antibody detects endogenous levels of Cdc25A protein only when phosphorylated at S75.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Cdc25A antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human Cdc25A (phospho Ser75)
Modification
Phospho Specific
Preparation and Storage
Store at -20 degree C/1 year
Application Data
(Li, Lin, et al. "Telekin suppresses human hepatocellular carcinoma cells in vitro by inducing G 2/M phase arrest via the p38 MAPK signaling pathway." Acta Pharmacologica Sinica 35.10 (2014): 1311.)
Application Data
(Li, Lin, et al. "Telekin suppresses human hepatocellular carcinoma cells in vitro by inducing G 2/M phase arrest via the p38 MAPK signaling pathway." Acta Pharmacologica Sinica 35.10 (2014): 1311.)
WB (Western Blot)
(Western Blot analysis of various cells using Phospho-Cdc25A (S75) Polyclonal Antibody)
WB (Western Blot)
(Western Blot analysis of various cells using Phospho-Cdc25A (S75) Polyclonal Antibody)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast cancer, using CDC25A (Phospho-Ser75) Antibody. The picture on the right is blocked with the CDC25A (Phospho-Ser75) peptide.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast cancer, using CDC25A (Phospho-Ser75) Antibody. The picture on the right is blocked with the CDC25A (Phospho-Ser75) peptide.)
WB (Western Blot)
(Western blot analysis of CDC25A (Phospho-Ser75) Antibody. The lane on the right is blocked with the CDC25A (Phospho-Ser75) peptide.)
WB (Western Blot)
(Western blot analysis of CDC25A (Phospho-Ser75) Antibody. The lane on the right is blocked with the CDC25A (Phospho-Ser75) peptide.)
Related Product Information for anti-Cdc25A antibody
cell division cycle 25A (CDC25A) Homo sapiens CDC25A is a member of the CDC25 family of phosphatases. CDC25A is requiredfor progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,087 Da
NCBI Official Full Name
M-phase inducer phosphatase 1 isoform a
NCBI Official Synonym Full Names
cell division cycle 25A
NCBI Official Symbol
CDC25A
NCBI Official Synonym Symbols
CDC25A2
NCBI Protein Information
M-phase inducer phosphatase 1; CDC25A2-CAG isoform; dual specificity phosphatase CDC25A
UniProt Protein Name
M-phase inducer phosphatase 1
UniProt Gene Name
CDC25A
UniProt Entry Name
MPIP1_HUMAN
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Product Notes
The Cdc25A cdc25a (Catalog #AAA318643) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cdc25A (Phospho-Ser75) Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cdc25A can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Cdc25A cdc25a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdc25A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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