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FCM/FACS (Flow Cytometry)
(CDC42EP3 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit CDC42EP3 Polyclonal Antibody | anti-CDC42EP3 antibody
CDC42EP3 Antibody (N-term)
Gene Names
CDC42EP3; UB1; CEP3; BORG2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CDC42EP3, Antibody; CDC42EP3 Antibody (N-term); Cdc42 effector protein 3; Binder of Rho GTPases 2; MSE55-related Cdc42-binding protein; CDC42EP3; BORG2; CEP3; anti-CDC42EP3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CDC42EP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CDC42EP3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1-30
Sequence Length
254
Applicable Applications for anti-CDC42EP3 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CDC42EP3 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CDC42EP3 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of CDC42EP3 (arrow) using rabbit polyclonal CDC42EP3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC42EP3 gene.)
WB (Western Blot)
(Western blot analysis of CDC42EP3 (arrow) using rabbit polyclonal CDC42EP3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC42EP3 gene.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with CDC42EP3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with CDC42EP3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of CDC42EP3 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDC42EP3 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CDC42EP3 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDC42EP3 (arrow) was detected using the purified Pab.)
Related Product Information for anti-CDC42EP3 antibody
CDC42EP3 is probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. It induces pseudopodia formation in fibroblasts.
Product Categories/Family for anti-CDC42EP3 antibody
References
Simpson,J.C., et.al., EMBO Rep. 1 (3), 287-292 (2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27678
NCBI Official Full Name
cdc42 effector protein 3
NCBI Official Synonym Full Names
CDC42 effector protein (Rho GTPase binding) 3
NCBI Official Symbol
CDC42EP3
NCBI Official Synonym Symbols
UB1; CEP3; BORG2
NCBI Protein Information
cdc42 effector protein 3
UniProt Protein Name
Cdc42 effector protein 3
UniProt Gene Name
CDC42EP3
UniProt Synonym Gene Names
BORG2; CEP3
UniProt Entry Name
BORG2_HUMAN
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Product Notes
The CDC42EP3 cdc42ep3 (Catalog #AAA287486) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The CDC42EP3 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's CDC42EP3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the CDC42EP3 cdc42ep3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC42EP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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