IHC (Immunohiostchemistry)
(AAA326541 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit CDC45 Polyclonal Antibody | anti-CDC45 antibody
CDC45 Antibody
Gene Names
CDC45; CDC45L; MGORS7; CDC45L2; PORC-PI-1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CDC45, Antibody; CDC45 Antibody; CDC 45; CDC 45L; CDC45 (cell division cycle 45, S.cerevisiae homolog) like; CDC45; CDC45 cell division cycle 45 like; CDC45 like; CDC45 related protein; CDC45_HUMAN; CDC45L2; Cell division control protein 45 homolog; Cell division cycle 45 like 2; Cell division cycle 45 like; PORC PI 1; PORC-PI-1; anti-CDC45 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
cdc45 antibody detects endogenous levels of total cdc45
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
598
Applicable Applications for anti-CDC45 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human cdc45
Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog, Chicken
Similarity
Pig (92%), Bovine (92%), Horse (92%), Sheep (92%), Dog (100%), Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA326541 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA326541 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of Hela whole cell lysates, using cdc45 Antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of Hela whole cell lysates, using cdc45 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-CDC45 antibody
Description: The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
Function: Required for initiation of chromosomal DNA replication.
Subunit Structure: Associated with ORC2. Interacts with HELB (PubMed:25933514).
Similarity: Belongs to the CDC45 family.
Function: Required for initiation of chromosomal DNA replication.
Subunit Structure: Associated with ORC2. Interacts with HELB (PubMed:25933514).
Similarity: Belongs to the CDC45 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 64 kDa
Predicted: 66 kDa
Predicted: 66 kDa
NCBI Official Full Name
cell division control protein 45 homolog isoform 1
NCBI Official Synonym Full Names
cell division cycle 45
NCBI Official Symbol
CDC45
NCBI Official Synonym Symbols
CDC45L; MGORS7; CDC45L2; PORC-PI-1
NCBI Protein Information
cell division control protein 45 homolog
UniProt Protein Name
Cell division control protein 45 homolog
UniProt Gene Name
CDC45
UniProt Synonym Gene Names
CDC45L; CDC45L2
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Product Notes
The CDC45 cdc45 (Catalog #AAA326541) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC45 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDC45 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CDC45 cdc45 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC45, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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