FCM/FACS (Flow Cytometry)
(Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-CDCA5 antibody (bs-7717R) Dilution: 3 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 3 ug /test.Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
Rabbit CDCA5 Polyclonal Antibody | anti-CDCA5 antibody
CDCA5 Polyclonal Antibody
Gene Names
CDCA5; SORORIN
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CDCA5, Antibody; CDCA5 Polyclonal Antibody; Sororin; Cell division cycle-associated protein 5; p35; CDCA5; anti-CDCA5 antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-CDCA5 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-CDCA5 antibody (bs-7717R) Dilution: 3 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 3 ug /test.Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-CDCA5 antibody (bs-7717R) Dilution: 3 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 3 ug /test.Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
IHC (Immunohistochemistry)
(Tissue/cell: Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation:Anti-CDCA5/Sororin Polyclonal Antibody, Unconjugated(bs-7717R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IHC (Immunohistochemistry)
(Tissue/cell: Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation:Anti-CDCA5/Sororin Polyclonal Antibody, Unconjugated(bs-7717R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,601 Da
NCBI Official Full Name
sororin
NCBI Official Synonym Full Names
cell division cycle associated 5
NCBI Official Symbol
CDCA5
NCBI Official Synonym Symbols
SORORIN
NCBI Protein Information
sororin; p35; cell division cycle-associated protein 5
UniProt Protein Name
Sororin
UniProt Gene Name
CDCA5
UniProt Entry Name
CDCA5_HUMAN
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Product Notes
The CDCA5 cdca5 (Catalog #AAA292086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDCA5 Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CDCA5 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CDCA5 cdca5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDCA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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