IF (Immunofluorescence)
(AAA328547 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328547 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat antimouse IgG(H+L) Ab(Green) were used as the secondary)
Rabbit anti-Human CDCP1 Polyclonal Antibody | anti-CDCP1 antibody
Phospho-CDCP1 (Tyr734) Antibody
Gene Names
CDCP1; CD318; TRASK; SIMA135
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Peptide affinity purification
Synonyms
CDCP1, Antibody; Phospho-CDCP1 (Tyr734) Antibody; 9030022E12Rik; AA409659; CD 318; CD_antigen=CD318; CD318; CD318 antigen; CDCP 1; Cdcp1; CDCP1_HUMAN; CUB domain containing protein 1; CUB domain-containing protein 1; E030027H19Rik; FLJ13772; FLJ22375; FLJ22969; Membrane glycoprotein gp140; MGC31813; OTTHUMP00000164120; OTTHUMP00000209568; RGD1305578; SIMA 135; SIMA135; Subtractive immunization M plus HEp3 associated 135 kDa protein; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases; TRASK; anti-CDCP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-CDCP1 (Tyr734) Antibody detects endogenous levels of CDCP1 only when phosphorylated at Tyr734.
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Sequence Length
836
Applicable Applications for anti-CDCP1 antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CDCP1 around the phosphorylation site of Tyr734.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328547 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328547 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat antimouse IgG(H+L) Ab(Green) were used as the secondary)
IF (Immunofluorescence)
(AAA328547 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328547 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat antimouse IgG(H+L) Ab(Green) were used as the secondary)
WB (Western Blot)
(Western blot analysis of Phospho-CDCP1 (Tyr734) in lysates of COLO205, using Phospho-CDCP1 (Tyr734) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-CDCP1 (Tyr734) in lysates of COLO205, using Phospho-CDCP1 (Tyr734) Antibody.)
Related Product Information for anti-CDCP1 antibody
Description: May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia identification and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
90kDa, 120kDa
NCBI Official Full Name
CUB domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
CUB domain containing protein 1
NCBI Official Symbol
CDCP1
NCBI Official Synonym Symbols
CD318; TRASK; SIMA135
NCBI Protein Information
CUB domain-containing protein 1
UniProt Protein Name
CUB domain-containing protein 1
UniProt Gene Name
CDCP1
UniProt Synonym Gene Names
TRASK; SIMA135
UniProt Entry Name
CDCP1_HUMAN
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Product Notes
The CDCP1 cdcp1 (Catalog #AAA328547) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CDCP1 (Tyr734) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDCP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the CDCP1 cdcp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDCP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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