FCM/FACS (Flow Cytometry)
(Dilution: FC: 1:10~50Flow cytometric analysis of 293 cells using CDH2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit CDH2 Polyclonal Antibody | anti-CDH2 antibody
CDH2 Antibody (C-term)
Gene Names
CDH2; CDHN; NCAD; CD325; CDw325
Reactivity
HumanPredicted Reactivity: Xenopus, Bovine, Chicken, Zebrafish, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CDH2, Antibody; CDH2 Antibody (C-term); Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD325; CDH2; CDHN; NCAD; anti-CDH2 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Xenopus, Bovine, Chicken, Zebrafish, Mouse, Rat
Predicted Reactivity: Xenopus, Bovine, Chicken, Zebrafish, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This CDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 744-772 amino acids from the C-terminal region of human CDH2.
Purity/Purification
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-CDH2 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Antigen Region
744-772
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Dilution: FC: 1:10~50Flow cytometric analysis of 293 cells using CDH2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Dilution: FC: 1:10~50Flow cytometric analysis of 293 cells using CDH2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Dilution: WB 1:1000Western blot analysis of CDH2 (arrow) using rabbit polyclonal CDH2 Antibody (C-term) (Cat.#AAA291026).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH2 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Dilution: WB 1:1000Western blot analysis of CDH2 (arrow) using rabbit polyclonal CDH2 Antibody (C-term) (Cat.#AAA291026).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH2 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Dilution: WB 1:1000Western blot analysis of CDH2 Antibody (C-term) (Cat.#AAA291026) in 293 cell line lysates (35ug/lane). CDH2(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Dilution: WB 1:1000Western blot analysis of CDH2 Antibody (C-term) (Cat.#AAA291026) in 293 cell line lysates (35ug/lane). CDH2(arrow) was detected using the purified Pab.)
Related Product Information for anti-CDH2 antibody
CDH2 is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
Product Categories/Family for anti-CDH2 antibody
References
Reid R.A., Nucleic Acids Res. 18:5896-5896 (1990).Salomon D., J. Cell Sci. 102:7-17 (1992).Amanchy, R., J. Proteome Res. 4 (5), 1661-1671 (2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
99,809 Da
NCBI Official Full Name
cadherin-2 preproprotein
NCBI Official Synonym Full Names
cadherin 2, type 1, N-cadherin (neuronal)
NCBI Official Symbol
CDH2
NCBI Official Synonym Symbols
CDHN; NCAD; CD325; CDw325
NCBI Protein Information
cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal
UniProt Protein Name
Cadherin-2
UniProt Gene Name
CDH2
UniProt Synonym Gene Names
CDHN; NCAD; N-cadherin
UniProt Entry Name
CADH2_HUMAN
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Product Notes
The CDH2 cdh2 (Catalog #AAA291026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDH2 Antibody (C-term) reacts with Human Predicted Reactivity: Xenopus, Bovine, Chicken, Zebrafish, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDH2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the CDH2 cdh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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