Rabbit CDH20 Polyclonal Antibody | anti-CDH20 antibody

CDH20 Antibody (N-term)

Cat. Num. AAA289721

• Gene Names: CDH20; Cdh7; CDH7L3
• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Synonyms: CDH20, Antibody; CDH20 Antibody (N-term); Cadherin-20; CDH20; CDH7L3; anti-CDH20 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CDH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human CDH20.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Applicable Applications for anti-CDH20 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Region: 111-140
• Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
• Cellular Location: Cell membrane; Single-pass type I membrane protein
• Tissue Location: Expressed in placenta, adult brain, and fetal brain
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(CDH20 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemisry)

(CDH20 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CDH20 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of CDH20 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). CDH20 (arrow) was detected using the purified Pab.)

WB (Western Blot)

(Western blot analysis of lysates from human brain and mouse brain tissue lysate(from left to right), using CDH20 Antibody (N-term). AAA289721 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Related Product Information for anti-CDH20 antibody

This gene is a type II classical cadherin from the cadherin superfamily and one of three cadherin 7-like genes located in a cluster on chromosome 18. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Since disturbance of intracellular adhesion is a prerequisite for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes.

Product Categories/Family for anti-CDH20 antibody

Crown Antibodies; Signal Transduction

References

Kools, P., et al. Genomics 68(3):283-295(2000)

NCBI and Uniprot Product Information

• NCBI GI #: 16306537
• NCBI GeneID: 28316
• NCBI Accession #: NP_114097.2
• NCBI GenBank Nucleotide #: NM_031891.2
• UniProt Accession #: Q9HBT6; Q495S3
• Molecular Weight: 88993
• NCBI Official Full Name: cadherin-20 preproprotein
• NCBI Official Synonym Full Names: cadherin 20, type 2
• NCBI Official Symbol: CDH20
• NCBI Official Synonym Symbols: Cdh7; CDH7L3
• NCBI Protein Information: cadherin-20
• UniProt Protein Name: Cadherin-20
• UniProt Gene Name: CDH20
• UniProt Synonym Gene Names: CDH7L3
• UniProt Entry Name: CAD20_HUMAN