IP (Immunoprecipitation)
(CDK1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg A431 Whole Cell Lysate4 uL anti-CDK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CDK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)
Rabbit anti-Human CDK1 Polyclonal Antibody | anti-CDK1 antibody
Anti-CDK1 Antibody, Rabbit Polyclonal
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CDK1, Antibody; Anti-CDK1 Antibody, Rabbit Polyclonal; CDC2 Kinase/CDK1 Antibody, Rabbit PAb, Antigen Affinity Purified; cyclin-dependent kinase 1; Anti-CDC2 Antibody; Anti-CDC28A Antibody; Anti-P34CDC2 Antibody; anti-CDK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CDC2 Kinase/CDK1
Purity/Purification
Antigen Affinity Purified
Form/Format
0.2um filtered solution in PBS
Applicable Applications for anti-CDK1 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human CDC2 Kinase/CDK1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CDC2 Kinase/CDK1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(CDK1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg A431 Whole Cell Lysate4 uL anti-CDK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CDK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)
IP (Immunoprecipitation)
(CDK1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg A431 Whole Cell Lysate4 uL anti-CDK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CDK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of CDK1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CDK1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of CDK1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CDK1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
WB (Western Blot)
(Anti-CDK1 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: SKBR3 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-CDK1 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: SKBR3 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
References
Lee MG, et al. (1987) Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature. 327(6117):31-5.Enserink JM, et al. (2010) An overview of Cdk1-controlled targets and processes. Cell Division. 5(11): 1-41.Ninomiya-Tsuji J, et al. (1991) Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation. Proc Natl Acad Sci. 88(20):9006-10.Zhan Q, et al. (1999) Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45. Oncogene. 18(18):2892-900.Jin S, et al. (2000) The GADD45 inhibition of Cdc2 kinase correlates with GADD45-mediated growth suppression. J Biol Chem. 275(22):16602-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cyclin-dependent kinase 1 isoform 1
NCBI Official Synonym Full Names
cyclin-dependent kinase 1
NCBI Official Symbol
CDK1
NCBI Official Synonym Symbols
CDC2; CDC28A; P34CDC2
NCBI Protein Information
cyclin-dependent kinase 1; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M
UniProt Protein Name
Cyclin-dependent kinase 1
UniProt Gene Name
CDK1
UniProt Synonym Gene Names
CDC2; CDC28A; CDKN1; P34CDC2; CDK1
UniProt Entry Name
CDK1_HUMAN
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Product Notes
The CDK1 cdk1 (Catalog #AAA254349) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CDK1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDK1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CDK1 cdk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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