IP (Immunoprecipitation)
(CDK1 was immunoprecipitated using:; Lane A:0.5 mg NIH3T3 Whole Cell Lysate; 4 uL anti-CDK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-CDK1 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 34 kDa; Observed band size :34 kDa)
Rabbit anti-Human CDK1 Polyclonal Antibody | anti-CDK1 antibody
CDK1 Antibody, Rabbit PAb, Antigen Affinity Purified
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Synonyms
CDK1, Antibody; CDK1 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-CDC2 Antibody; Anti-CDC28A Antibody; Anti-P34CDC2 Antibody; anti-CDK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human CDK1
Applicable Applications for anti-CDK1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CDK1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(CDK1 was immunoprecipitated using:; Lane A:0.5 mg NIH3T3 Whole Cell Lysate; 4 uL anti-CDK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-CDK1 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 34 kDa; Observed band size :34 kDa)
IP (Immunoprecipitation)
(CDK1 was immunoprecipitated using:; Lane A:0.5 mg NIH3T3 Whole Cell Lysate; 4 uL anti-CDK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-CDK1 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 34 kDa; Observed band size :34 kDa)
WB (Western Blot)
(Anti-CDK1 rabbit polyclonal antibody at 1:500 dilution; Lane A:HepG2 Whole Cell Lysate; Lane B: HeLa Whole Cell Lysate; Lane C: Jurkat Whole Cell Lysate; Lane D: NIH-3T3 Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:34 kDa; Observed band size:34 kDa)
WB (Western Blot)
(Anti-CDK1 rabbit polyclonal antibody at 1:500 dilution; Lane A:HepG2 Whole Cell Lysate; Lane B: HeLa Whole Cell Lysate; Lane C: Jurkat Whole Cell Lysate; Lane D: NIH-3T3 Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:34 kDa; Observed band size:34 kDa)
Related Product Information for anti-CDK1 antibody
CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.
Product Categories/Family for anti-CDK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,095 Da
NCBI Official Full Name
cyclin-dependent kinase 1 isoform 1
NCBI Official Synonym Full Names
cyclin-dependent kinase 1
NCBI Official Symbol
CDK1
NCBI Official Synonym Symbols
CDC2; CDC28A; P34CDC2
NCBI Protein Information
cyclin-dependent kinase 1; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M
UniProt Protein Name
Cyclin-dependent kinase 1
UniProt Gene Name
CDK1
UniProt Synonym Gene Names
CDC2; CDC28A; CDKN1; P34CDC2; CDK1
UniProt Entry Name
CDK1_HUMAN
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Product Notes
The CDK1 cdk1 (Catalog #AAA258625) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDK1 Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDK1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CDK1 cdk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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